关键词: 激活素受体, Ⅱ型, 伴刀豆球蛋白A, 免疫性肝损伤

Abstract: Abstract:Objective To investigate the expression pattern of activin and type ⅡA receptor of activin (ActRⅡA) and its possible effect on liver injury of Con A-induced model mice.Methods 24 mice were injected with Con A (10 mg•kg^-1) in the tail vein weekly for 4 consecutive weeks to cause liver injury and normal control mice (n=24) were used as control.Then the levels of serum ALT and AST and severity of liver injury were examined, as well as the expression levels of activin and ActR ⅡA mRNA in liver were detected by using fluorescent quantitive PCR. Results The liver injury was most obvious at 72 h after the last injection, such as structure of liver lobules disordered, hepatic cord disappeared, balloon-like hepatocytes swelled, and then gradually repaired from 120 h to 168 h.The activin A protein level and its mRNA expression paralleled with ActRⅡA mRNA expression, and also reached the peak at 72 h after the last injection, which had significant difference compared with control mice (P<0.05).Conclusion The changes of activin A and ActR ⅡA are positively correlated with the severity of liver pathological changes, which indicate that activin may take part in Con A-induced mouse liver injury.

Key words: activin receptors, type Ⅱ, concanavalin A, immunological liver injury