关键词: 前列腺肿瘤, 凋亡, 细胞周期

Abstract: To investigate the inhibitory effects of polyI∶C on growth of PC-3M cell lines. Methods　he prostate cancer PC-3M cells were observed by contrast phase microscope after being treated with different doses of PolyI∶C（100,200,400 μg•L^-1）.The cell growth inhibitory rate was detected by 　TT assay, and content of DNA was detected by flow cytometry, as well as the cell cycle and the apoptosis. Results　he cells in control group were fusiform adherence and abundant, the state of cells in Poly I∶C group was worse than that in control group.The inhibitory rates of growth in Poly I∶C with different doses(400,200,100 μg•L^-1)groups were 67.16%,52.84% and 42.00%,respectively,there were significant differences between three groups(P<0.05).The inhibitory rate in Poly I∶C group with the same dose at 72 h was higher than those at 48 and 24 h,there were significant differences between three groups(P<0.05). The result of flow cytometry showed that 　oly I∶C could induce the apoptosis with dose-dependent manner,and the apoptotic rates were 3.9%，27.1%，42.9%，respectively,there were significant differences between three groups(P<0.05) The G₁ phase cell proportion in PolyI∶C group(61.4%) was higher than that in control group（33.9%）. Conclusion　olyI∶C has significant effect on PC-3M cell inhibition and the inhibitory rate is dose and time dependent.The mechanism may be concerned with the cell cycle arrest and inducing apoptosis upon the PC-3M cells

Key words: prostate cancer , apoptosis , cell cycle