关键词: Jurkat细胞, 细胞凋亡

Abstract: Objective To investigate the effect of resveratrol on proliferation inhibition, cell cycle arrest and apoptosis of Jurkat cell line in acute T lymphoblast leukemia. Methods MTT assay was used to determine the cell vitality. Wright-Giemsa，Hoechest 33258/PI staining and transmission electron microscope technique were used to detect the apoptosis status of Jurkat cells. The cell cycle arrest was analyzed by flow cytometry. Results Resveratrol had 64.01% inhibitory rate on the growth of Jurkat cells at 0.2 mmol·L^-1 and inhibited the growth of Jurkat cells in dose- and time-dependent manner. 24 h after treated with resveratrol,the typical features of apoptosis were observed under light and electron microscope in all treatment groups. Some nuclei showed bright blue under fluorescence microscope in the resveratrol-treated Jurkat cells, and the number of cells with bright blue fluorescence increased with time. Nuclei condensation and fragmentation were observed. Cell shrinkage, chromatin condensation, and marginalization were found by Wrigh-Giemsa staining and transmission electron microscope technique. By flow cytometry, 62.57% of the cells were arrested at the S phase after exposured to 0.05 mmol·L^-1 resveratrol for 48 h, the rate of apoptotic cells to total cells was 12.01% in 0.05 mmol·L^-1 treatment groups, and that in the control groups was 2.05%. Conclusion Resveratrol can inhibit the proliferation, cause S-phage arrest and induce the apoptosis of Jurkat cells.

Key words: Jurkat cells, apoptosis