J4

• 临床研究 • 上一篇    下一篇

核心蛋白聚糖mRNA及其蛋白在结直肠癌中的表达

操海萍1△, 舒振波2,张桂珍1*   

  1. 1. 吉林大学中日联谊医院中心实验室,吉林 长春 130033;2. 吉林大学中日联谊医院普通外科,吉林 长春 130033
  • 收稿日期:2005-04-21 修回日期:1900-01-01 出版日期:2006-03-28 发布日期:2006-03-28
  • 通讯作者: 张桂珍1*

Expressions of decorin mRNA and protein in colorectal carcinoma

CAO Hai-ping1△,SU Zhen-bo2,ZHANG Gui-zhen1*   

  1. 1. Department of Central Laboratory,China-Japan Union Hospital,Jilin University,Changchun 130033, China;2. Department of General Surgery,China-Japan Union Hospital,Jilin University, Changchun 130033,China
  • Received:2005-04-21 Revised:1900-01-01 Online:2006-03-28 Published:2006-03-28
  • Contact: ZHANG Gui-zhen1*

摘要: 目的:研究核心蛋白聚糖(DCN)mRNA及其蛋白在结直肠癌中的表达。方法:采用原位杂交及免疫组织化学方法检测DCN mRNA及其蛋白在30例结直肠癌及20例相对正常大肠组织中的表达。结果:30例结直肠癌患者,DCN mRNA表达阳性率为66.67%(20/30); 20例相对正常大肠腺细胞中,DCN mRNA表达阳性率为85.00%(17/20),两者比较差异无显著性(P>0.05)。在30例结直肠癌癌细胞及20例相对正常大肠腺细胞内未发现DCN蛋白表达。结论:DCN mRNA在结直肠癌癌细胞及相对正常大肠腺细胞中均有表达。

关键词: 核心蛋白聚糖, 原位杂交, 免疫组织化学, 方法

Abstract: Objective To investigate the expressions of decorin (DCN) mRNA and protein in colorectal carcinoma tissues. Methods Immunohistochemistry and in situ hybridization technique were used to detect DCN protein and mRNA expressions in 30 cases of colorectal carcinoma tissues and 20 cases of relative normal tissues. Results The positive expression rate of DCN mRNA in 30 cases of colorectal carcinoma was 66.67%(20/30), while the positive expression rate of DCN mRNA in 20 cases of relative normal tissue was 85.00%(17/20). The difference between them was not significant (P>0.05). DCN protein didn′t express in both 20 cases of relative normal tissue and 30 cases of colorectal carcinoma. Conclusion DCN mRNA can express in both colorectal carcinoma and relative normal tissue.

Key words: decorin, in situ hybridization, immunohistochemistry, methods

中图分类号: 

  • R735.35