组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20170620

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (06): 1177-1181.doi: 10.13481/j.1671-587x.20170620

组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义

曹艳莎¹, 李婳¹, 陈明红², 刘宝琴², 王华芹², 李宁¹

1. 锦州医科大学生物化学教研室, 辽宁锦州 121000;
2. 中国医科大学生物化学教研室, 辽宁沈阳 110000

收稿日期:2017-03-13 出版日期:2017-11-28 发布日期:2017-12-01
通讯作者: 李宁,副教授,硕士研究生导师(Tel:0416-4673253,E-mail:157026753@qq.com) E-mail:157026753@qq.com
作者简介:曹艳莎(1989-),女,河北省邯郸市人,在读医学硕士,主要从事结肠肿瘤基础方面的研究。
基金资助:
国家自然科学基金资助课题（31400646）；辽宁省科技厅科研基金资助课题（201602293）

Expression of BAG3 protein in colon cancer tissue detected by tissue microarray method and its clinical significance

CAO Yansha¹, LI Hua¹, CHEN Minghong², LIU Baoqin², WANG Huaqin², LI Ning¹

1. Department of Biochemistry, Jinzhou Medical University, Jinzhou 121000, China;
2. Department of Biochemistry, Chinese Medical University, Shenyang 110000, China

Received:2017-03-13 Online:2017-11-28 Published:2017-12-01

摘要/Abstract

摘要： 目的：探讨Bcl相关抗凋亡蛋白3（又称BAG3蛋白）在结肠癌组织中的表达及其临床意义，阐明BAG3蛋白与结肠癌发生发展的关系。方法：选取90例结肠癌患者的结肠癌组织和癌旁组织，采用组织芯片技术和免疫组织化学SP法检测组织中BAG3蛋白的表达，Kaplan-Meier、单因素和多因素生存分析法分析结肠癌患者临床病理参数与预后的关系。结果：BAG3蛋白在结肠癌组织中呈高表达，90例结肠癌组织中BAG3蛋白阳性表达率为37.8%，癌旁组织中BAG3蛋白阳性表达率为0.01%，两者比较差异有统计学意义（P<0.05）。BAG3蛋白表达与患者性别和肿瘤大小有关联（P<0.05），与患者年龄、病理分级、TNM分期和有无淋巴结转移无关联（P>0.05）。Kaplan-Meier分析，BAG3在结肠癌组织中的表达与患者预后无关联，年龄较小的患者生存率较高，TNM分期低的患者生存率较高。结论：BAG3蛋白在结肠癌组织中呈高表达，蛋白表达与肿瘤大小有关联；BAG3蛋白可能作为结肠癌的肿瘤标志物或治疗靶点。

关键词: 结肠肿瘤, 组织芯片, 免疫组织化学, Bcl相关抗凋亡蛋白3

Abstract: Objective:To explore the expression of Bcl related anti-apoptotic protein3(BAG3 protein) in colon cancer and its clinical significance, and to clarify the relationship between its expression and the occurrence and development of colon cancer. Methods:A total of 90 cases of colon cancer tissue and paracancerous tissue were selected, the expressions of BAG3 protein in different tissues were detected by tissue microarray and immunohistochemical SP method; Kaplan-Meier method, univariate analysis and multivariate analysis were used to investigate the relationship between the clinicopathological parameters and the prognosis of the patients with colon cancer. Results:The expression of BAG3 protein in the colon cancer tissue was high,and the positive expression rates of BAG3 protein in the colon cancer tissue and paracancerous tissue were 37.8% and 0.01%, respectively,and the difference was significant(P < 0.05).The expression of BAG3 protein was associated with gender and tumor size(P < 0.05),but there was no correlation between the expression of BAG3 protein in the colon cancer tissue and age,pathologic grade,TNM stage,and lymph node metastasis (P > 0.05).The Kaplan-Meier results showed that the expression of BAG3 protein in the colon cancer tissue was not related with the prognosis of the patients and the survival rates of the younger patients and the higher TNM staging patients were high. Conclusion:The expression of BAG3 protein in the colon cancer tissue is high, and its expression is associated with the tumor size; BAG3 protein may act as a tumor marker and therapeutic target for colon cancer.

Key words: colorectal neoplasm, Bcl related anti-apoptotic protein 3, tissue microarray, immunohistochemistry

中图分类号:

R735.35

曹艳莎, 李婳, 陈明红, 刘宝琴, 王华芹, 李宁. 组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(06): 1177-1181.

CAO Yansha, LI Hua, CHEN Minghong, LIU Baoqin, WANG Huaqin, LI Ning. Expression of BAG3 protein in colon cancer tissue detected by tissue microarray method and its clinical significance[J]. Journal of Jilin University Medicine Edition, 2017, 43(06): 1177-1181.

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组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义

Expression of BAG3 protein in colon cancer tissue detected by tissue microarray method and its clinical significance

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