蒺藜皂苷对氧化应激PC12细胞内NF-κB水平的影响及其机制

J4 ›› 2010, Vol. 36 ›› Issue (6): 1035-1038.

蒺藜皂苷对氧化应激PC12细胞内NF-κB水平的影响及其机制

姜恩平, 李红, 杨世杰

(吉林大学基础医学院药理学教研室|吉林　长春　130021)

收稿日期:2010-07-07 出版日期:2010-11-28 发布日期:2010-11-28
通讯作者: 杨世杰 E-mail:(Tel: 0431-85619483, E-mail: jcyaoli@sina.com)
作者简介:姜恩平(1975-)|女|吉林省吉林市人|讲师|医学博士|主要从事心血管药理学研究。
基金资助:
国家自然科学基金资助课题(30472020)

Effect of gross saponin tribulus terrestris on NF-κB in PC12 cells injured by oxidative stress and its mechanisms

JIANG En-Ping, LI Hong, YANG Shi-Jie

(Department of Pharmacology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China)

Received:2010-07-07 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的：研究蒺藜皂苷(GSTT)对H2O2诱导大鼠肾上腺嗜铬细胞瘤细胞(PC12)缺氧损伤时细胞内核转录因子(NF-κB)含量的影响，并探讨其机制。方法: 将处于对数生长期的PC12细胞分为对照组、模型组、蒺藜皂苷高剂量组（10 mg·L^-1）和低剂量组（3 mg·L^-1）。用H2O2建立PC12细胞缺氧损伤模型，应用流式细胞术检测细胞凋亡率，并采用流式细胞仪、免疫组织化学方法及Western blotting等方法检测NF-κB蛋白表达水平。结果：与模型组比较，蒺藜皂苷高和低剂量组的PC12细胞凋亡率明显降低(P<0.05)；与蒺藜皂苷低剂量组比较，蒺藜皂苷高剂量组细胞凋亡率降低(P<0.05)；流式细胞仪及Western blotting方法检测，与模型组比较，蒺藜皂苷高和低剂量组PC12细胞内NF-κB蛋白表达明显增加(P<0.05)，蒺藜皂苷高剂量组与低剂量组比较差异无显著性(P>0.05)。免疫组织化学方法检测，与对照组比较，模型组中胞浆呈棕黄色颗粒的细胞数明显减少，NF-κB蛋白表达强度显著降低 (P<0.01)；蒺藜皂苷高和低剂量组中NF-κB呈阳性表达的细胞数明显高于模型组(P<0.05)。结论：蒺藜皂苷对H2O2诱导的PC12细胞损伤具有一定的保护作用，其保护作用机制可能与NF-κB的激活有关。

关键词: 蒺藜皂苷；核转录因子-κB；PC12细胞；细胞凋亡

Abstract:

Abstract:Objective To investigate the influence of gross saponin tribulus terrestris (GSTT) on nuclear factor-κB (NF-κB) of pheochromocytoma cells (PC12 cells) injured by hypoxia and its mechanisms. Methods PC12 cells were divided into control group, model group, high dose GSTT group（10 mg·L^-1） and low dose GSTT group（3 mg·L^-1）. The hypoxia model was set up with H2O2. The apoptotic rate of PC12 cells was detected by flow cytometry. The protein expression of NF-κB was detected by flow cytometry, immunohistochemistry and Western blotting methods. Results Compared with model group, the apoptotic rates of PC12 cells in high dose GSTT group and low dose GSTT group were significantly reduced (P<0.05). Compared with low dose GSTT group, the apoptotic rate of cells in high dose GSTT group was reduced (P<0.05). The results of flow cytometry and Western blotting showed that the NF-κB expressions in high dose GSTT group and low dose GSTT group were significantly increased compared with model group (P<0.05). There was no significant difference of the NF-κB expression between high dose GSTT group and l
ow dose GSTT group. The results of immunohistochemistry showed that few brown and black grains were found in the cytochylema in model group and the NF-κB expression was reduced compared with control group(P<0.01). Compared with model group, the NF-κB expressions in high dose GSTT group and low dose group were significantly increased (P<0.05). Conclusion GSTT can protect PC12 cells injured by hypoxia which might be correlated with the activation of nuclear factor-κB expression.

Key words: gross saponins tribulus terrestris , nuclear factor-κB , PC12 cells, apoptosis

中图分类号:

R285.5

姜恩平, 李红, 杨世杰. 蒺藜皂苷对氧化应激PC12细胞内NF-κB水平的影响及其机制[J]. J4, 2010, 36(6): 1035-1038.

JIANG En-Ping, LI Hong, YANG Shi-Jie. Effect of gross saponin tribulus terrestris on NF-κB in PC12 cells injured by oxidative stress and its mechanisms[J]. J4, 2010, 36(6): 1035-1038.

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