MMP-3、MMP-9及TIMP-1在急性心肌梗塞中的表达及其意义

doi:吉林省科技厅科研基金资助课题（20050408-3

MMP-3、MMP-9及TIMP-1在急性心肌梗塞中的表达及其意义

沙洪芳¹，秦玲¹，李洋¹，张家颖²

（1．吉林大学第一医院心内科，吉林长春 130021；2. 吉林大学基础医学院生物化学与分子生物学实验中心，吉林长春 130021）

收稿日期:2007-12-21 修回日期:1900-01-01 出版日期:2008-09-28 发布日期:2008-09-28
通讯作者: 秦玲

Expressions of MMP-3,MMP-9 and TIMP-1 in patients with acute 　myocardial infarction and their significances

SHA Hong-fang¹,QIN Ling¹,LI Yang¹，ZHANG Jia-ying²

（1. Department of Cardiology,First Hospital,Jiling University,Changchun 130021,China;2. Experiment Center of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Jiling University,Changchun 130021,China）

Received:2007-12-21 Revised:1900-01-01 Online:2008-09-28 Published:2008-09-28
Contact: QIN Ling

摘要/Abstract

摘要： 目的: 观察急性心肌梗塞（AMI）患者血清中基质金属蛋白酶-3（MMP-3）、基质金属蛋白酶-9（MMP-9）以及基质金属蛋白酶抑制剂-1（TIMP-1）水平的变化，探讨冠状动脉粥样硬化斑块破裂的机制。方法:选择67例AMI患者，发病3　h～5　d，选择同期住院非冠心病患者40例作为对照组，应用ELISA法测定血清MMP-3、MMP-9、TIMP-1含量以及血清肌钙蛋白Ⅰ（cTnⅠ）、肌酸激酶-MB（CK-MB）水平。结果：与对照组比较，AMI组血清中MMP-3、MMP-9和TIMP-1含量明显增高（P<0.05或P<0.01）；AMI组MMP-3/TIMP-1比值和MMP-9/TIMP-1比值均高于对照组（P<0.05）。MMP-3、MMP-9、TIMP-1的含量与cTnI、CK-MB水平无相关性。结论：MMP-3、MMP-9过度表达以及MMP-3/TIMP-1、MMP-9/TIMP-1比例失衡，参与冠状动脉粥样斑块的破裂和心肌梗塞的发生。

关键词: 基质金属蛋白酶-3, 基质金属蛋白酶-9, 基质金属蛋白酶抑制剂-1

Abstract: To study the levels of matrix metalloproteinase-3(MMP-3),matrix metalloproteinase-9(MMP-9) and inhibitor of metalloproteinases-1(TIMP-1) in patients with acute myocardial infarction(AMI) and mechanism of plaque rupture of coronary atherosclerosis.Methods Sixty-seven patients with AMI were selected,average invasion time was 3 h～5 d.40 cases of non-coronary heart disease were included in control group.The expressions of serum MMP-3,MMP-9,TIMP-1 and the levels of serum cTnI,CK-MB were analyzed by enzyme-linked immunosorbent assay. Results The serum MMP-3,MMP-9 and TIMP-1 levels in AMI group were higher than those in control group,there was significant difference between two groups（P<0.05 or P<0.01 ）.MMP-3/TIMP-1 and MMP-9/TIMP-1 in AMI group were higher than those in control group （P<0.05）.There was no correlation of serum MMP-3,MMP-9 and TIMP-1 with the levels of cTnI and CK-MB.Conclusion The overexpressions of MMP-3,MMP-9 and MMP-3/TIMP-1,MMP-9/TIMP-1 disorder may be involved in the procession of plaque rupture of coronary atherosclerosis and AMI.

Key words: matrix metalloproteinase-3, matrix metalloproteinase-9, inhibitor of metalloproteinases-1

中图分类号:

R543

沙洪芳，秦玲，李洋，张家颖. MMP-3、MMP-9及TIMP-1在急性心肌梗塞中的表达及其意义[J]. J4, 2008, 34(5): 860-863.

SHA Hong-fang,QIN Ling,LI Yang，ZHANG Jia-ying. Expressions of MMP-3,MMP-9 and TIMP-1 in patients with acute 　myocardial infarction and their significances[J]. J4, 2008, 34(5): 860-863.

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