关键词: 细胞凋亡, 细胞周期, 肿瘤, 鳞状细胞

Abstract: To detect the cell cycle change and the apoptosis at each phase of cell cycle in laryngeal carcinoma cells,and discuss the role of the cell cycle change and the apoptosis at each phase of cell cycle in the occurrence,development and prognosis of laryngeal carcinoma.Methods The TUNEL technique and flow cytometry(FCM) parameter analysis were used to detect the cell cycle change and the apoptosis at each phase of cell cycle in 15 cases of polyp of vocal cord and 387 patients with laryngeal carcinoma.Results Compared with the group of polyp of vocal cord,although the proportion of the cells at G₀G₁ phase increased in the group of laryngeal carcinoma,there was no significant difference(P>0.05).The apoptotic index (AI) in laryngeal carcinoma was obviously lower than that in polyp of vocal cord,and this phenomenon was mainly caused by the decrease of the apoptosis of the cells at G₀G₁ phase.The proportion of the cells at G₂M phase,the total AI,the AI at S phase and G₂M phase were obviously enhanced in the poor-differentiated cancer,and this result was also observed both in the group of recurrence and in the patients who died in 5 years after operation,there was no significant difference between the well-differentiated and the moderate-differentiated cancer.The proportion of the cells at G₂M phase,the total AI and the AI at G₀G₁ phase in the group of no-relapse and the patients who lived more than 5 years after operation were strongly lower than that in polyp of vocal cord(P>0.05).Conclusion The significant increasing of the proportion of the cells at G₂M phase,the total AI,the AI at S phase and G₂M phase could be regard as S indicators to judge prognosis and assistant therapy for laryngeal carcinoma.

Key words: cell cycle, apoptosis, neoplasms, squamous cells