关键词: 含硒桥联环糊精；H22小鼠肝癌细胞；细胞凋亡

Abstract:

Abstract:Objective  To study the  effects of 2-selenium bridged β-cyclodextrin (2-SeCD) on the apoptosis of H22 cell line and explore its activity of anti-tumor. Methods 2-SeCD with different doses (80,160,320,640  μmol·L^-1 ) 　were added into the medium in which the cells were cultivated  in vitro,   negative control group was set up also.The morphological changes of H22 cells were observed under phase contrast microscope;the inhibibory rate of the growth of H22 cells was detected with MTT method,IC₅₀ was calculated,the cell cycle and apoptosis of H22 cells were determined with flow cytometry.Results After exposure of H22 cells to 2-SeCD at the doses of 320 and 640  μmol·L^-1 for 48 h,the inhibitory rates of  H22 cells were （25.90±2.13）% and （49.40±3.66）%,they were higher than that in control group (P<0.01).2-SeCD inhibited the growth of  H22 cells in dose-dependent  manner,and its  IC₅₀ was 611.29  μmol·L^-1 . After exposure of H22 cells to 2-SeCD at the doses of 320 and  640  μmol·L^-1  for 48 h,the apoptotic rates were 5.52% and 12.47%,respectively. 2-SeCD  inhibited the proliferation of cell cycle at G₀／G₁ phase.Conclusion 2-SeCD can suppress the growth of  H22 cells and induce apoptosis,and it  has a potential treatment value in inhibiting hepatoma cell proliferation.

Key words: 2-selenium bridged β-cyclodextrin；H22 cell line；apoptosis