J4 ›› 2009, Vol. 35 ›› Issue (4): 604-607.

• 基础研究 • 上一篇    下一篇

含硒桥联环糊精诱导体外培养H22小鼠肝癌细胞凋亡的作用

许新1, 王冰梅1,2, 王微1, 潘宇2, 林婷婷2, 闫岗林2, 罗贵民2   

  1. 1.长春中医药大学研发中心|吉林 长春 130117;2.吉林大学分子酶学工程教育部重点实验室|吉林 长春 130021
  • 收稿日期:2009-02-23 出版日期:2009-07-28 发布日期:2009-08-24
  • 通讯作者: 罗贵民 E-mail:luogm@jlu.edu.cn
  • 作者简介:许 新(1954-)|男|吉林省长春市人|副研究员|主要从事心血管及肿瘤药理学研究。
  • 基金资助:

    国家自然科学基金资助课题(20572035)

Apoptosis of mouse H22 cell cultured in vitroinduced with 2-selenium bridged β-cyclodextrin

XU Xin1, WANG Bing-Mei1,2, WANG Wei1, BO Yu2, LIN Ting-Ting2, YAN Gang-Lin2, LUO Gui-Min2   

  1. 1.Department of Research and Development Center,Changchun University of Traditional Chinese Medicine,Changchun 130117|China;2.Key Laboratory for Molecular Enzymology and Engineering,Ministry of Education,Jilin University,Changchun 130021|China
  • Received:2009-02-23 Online:2009-07-28 Published:2009-08-24

摘要:

目的:研究含硒桥联环糊精(2-SeCD)对体外培养H22小鼠肝癌细胞凋亡的促进作用,探讨2-SeCD的抗肿瘤活性。方法:体外培养的H22细胞分别加入80、160、320和640 μmol·L-1  2-SeCD作为实验组,并设阴性对照组。倒置相差显微镜观察细胞形态的变化;MTT法检测细胞生长抑制率,计算中效抑制浓度(IC50);流式细胞术(FCM)检测细胞周期和凋亡。结果:320和640 μmol·L-1 2-SeCD组细胞生长抑制率分别为(25.90±2.13)%和(49.40±3.66)%,与对照组比较差异有显著性(P<0.01),2-SeCD 48 h的IC50为611.29  μmol·L-1 ;320和640  μmol·L-1 2-SeCD组凋亡率分别为5.52%和12.47%,2-SeCD可使细胞阻滞于G0/G1期。结论:2-SeCD可抑制H22细胞增殖并促进其凋亡,2-SeCD对肝癌具有潜在的治疗价值。

关键词: 含硒桥联环糊精;H22小鼠肝癌细胞;细胞凋亡

Abstract:

Abstract:Objective  To study the  effects of 2-selenium bridged β-cyclodextrin (2-SeCD) on the apoptosis of H22 cell line and explore its activity of anti-tumor. Methods 2-SeCD with different doses (80,160,320,640  μmol·L-1 )  were added into the medium in which the cells were cultivated  in vitro,   negative control group was set up also.The morphological changes of H22 cells were observed under phase contrast microscope;the inhibibory rate of the growth of H22 cells was detected with MTT method,IC50 was calculated,the cell cycle and apoptosis of H22 cells were determined with flow cytometry.Results After exposure of H22 cells to 2-SeCD at the doses of 320 and 640  μmol·L-1 for 48 h,the inhibitory rates of  H22 cells were (25.90±2.13)% and (49.40±3.66)%,they were higher than that in control group (P<0.01).2-SeCD inhibited the growth of  H22 cells in dose-dependent  manner,and its  IC50 was 611.29  μmol·L-1 . After exposure of H22 cells to 2-SeCD at the doses of 320 and  640  μmol·L-1  for 48 h,the apoptotic rates were 5.52% and 12.47%,respectively. 2-SeCD  inhibited the proliferation of cell cycle at G0/G1 phase.Conclusion 2-SeCD can suppress the growth of  H22 cells and induce apoptosis,and it  has a potential treatment value in inhibiting hepatoma cell proliferation.

Key words: 2-selenium bridged β-cyclodextrin;H22 cell line;apoptosis

中图分类号: 

  • R735.7