乳酸盐腹膜透析液对人腹膜间皮细胞凋亡及bcl-2、bax表达和caspase-3活性的影响

J4 ›› 2009, Vol. 35 ›› Issue (3): 470-473.

乳酸盐腹膜透析液对人腹膜间皮细胞凋亡及bcl-2、bax表达和caspase-3活性的影响

崔明姬¹|王芳²|顾春梅¹|黄金杰¹|南光贤³

1.吉林大学第一医院肾病科|吉林长春 130021;2.吉林大学药学院生物工程教研室|吉林长春 130021;3.吉林大学中日联谊医院脑血管病介入科|吉林长春 130033

收稿日期:2008-11-06 出版日期:2009-05-28 发布日期:2009-08-14
通讯作者: 南光贤 E-mail:ngx041550@yahoo.com.cn
作者简介:崔明姬（1964-）|女|吉林省长春市人|副教授|医学博士|主要从事慢性肾脏病防治及血液净化研究。
基金资助:
吉林省科技厅科研基金资助课题(200505197)

Effects of lactate peritoneal dialysis solution on apoptosis of human peritoneal mesothelial cells,expressions of bcl-2,bax and activity of caspase-3

CUI Meng-Ji¹, WANG Fang², GU Chun-Mei¹, HUANG Jin-Jie¹, NA Guang-Xian³

1.Department of Nephrology,First Hospital,Jilin University,Changchun 130021,China;2.Department of Biological Engineering,School of |Pharmacy,Jilin University,Chanchun 130021,China;3.Department of Cerebral Interventional Radiology,China-Union Hospital,Jilin University,Changchun 130033,China

Received:2008-11-06 Online:2009-05-28 Published:2009-08-14

摘要/Abstract

摘要：

目的：探讨不同浓度乳酸盐腹膜透析液(L-PDS)对腹膜间皮细胞(HPMC)凋亡和bcl-2、bax表达及caspase-3活性的影响。方法:采用酶消化法从人腹膜组织中分离间皮细胞，建立稳定的体外培养模型，分别用浓度为1.50%、2.50%和4.25%的L-PDS与HPMC共同培养，同时设对照组。采用流式细胞术检测HPMC凋亡，采用RT-PCR法测定bcl-2及bax mRNA的表达,采用免疫荧光法检测caspase-3的活性。结果:与对照组比较,L-PDS各组HPMC凋亡率增加，bcl-2 mRNA的表达降低，bax mRNA表达升高，caspase-3活性增加，上述指标变化中2.50%及4.25%L-PDS组与对照组比较差异有显著性(P<0.05)，4.25%L-PDS组与2.50%L-PDS组比较差异也有显著性(P<0.05)，而1.50%L-PDS组与对照组比较差异无显著性(P>0.05)。结论： L-PDS可诱导HPMC 凋亡，其机制可能是通过对bcl-2及bax mRNA表达的改变以及激活caspase-3活性而实现。

关键词: 乳酸盐腹膜透析液;腹膜间皮细胞;细胞凋亡;基因, bcl-2；bax； caspase-3

Abstract:

Abstract:Objective To study the effects of lactate peritoneal dialysis solution(L-PDS) with different concentrations on apoptosis of human peritoneal mesothelial cells(HPMC),the expressions of bcl-2,bax and activity of caspase-3.Methods HPMC were separated using enzyme digestion and cultivated stably in vitro.After HPMC were co-cultivated with different concentrations(1.50%,2.50%,4.25%) L-PDS,flow cytometry was used to test the apoptosis of HPMC,RT-PCR was used to observe the expressions of bcl-2 and bax,fluorometric method was used to detect the activity of caspase-3.
Results Compared with control group, L-PDS could induce the apoptosis of HPMC,especially in high concentration.With the increasing of L-PDS concentration,the expression of bcl-2 mRNA decreased,the expression of bax mRNA increased,the activity of caspase-3 raised.There were significant differences of the indexes mentioned above between 4.25%, 2.50% L-PDS groups and control group（P<0.05），there also was significant difference between 4.25% L-PDS group and 2.50% L-PDS group(P<0.05)，but there was no significant difference between 1.5% L-PDS group and control group(P>0.05).
Conclusion L-PDS could induce HPMC apopto9sis,which may be executed by alternating of the expressions of bcl-2,bax and activating of caspase-3.

Key words: lactate peritoneal dialysis solution;human peritoneal mesothelial cells;apoptosis;genes,bcl-2;bax;caspase-3

中图分类号:

R459.5

崔明姬, 王芳, 顾春梅, 黄金杰, 南光贤. 乳酸盐腹膜透析液对人腹膜间皮细胞凋亡及bcl-2、bax表达和caspase-3活性的影响[J]. J4, 2009, 35(3): 470-473.

CUI Meng-Ji, WANG Fang, GU Chun-Mei, HUANG Jin-Jie, NA Guang-Xian. Effects of lactate peritoneal dialysis solution on apoptosis of human peritoneal mesothelial cells,expressions of bcl-2,bax and activity of caspase-3[J]. J4, 2009, 35(3): 470-473.