羧甲基壳聚糖银对实验性大鼠牙周炎的治疗作用

J4 ›› 2009, Vol. 35 ›› Issue (4): 669-672.

羧甲基壳聚糖银对实验性大鼠牙周炎的治疗作用

付军权¹, 费瑞¹, 张桂荣², 迟立超¹, 王书香¹, 佟巨慧³

1.吉林大学基础医学院细胞生物学教研室|吉林长春 130021；2.吉林大学生命科学学院生物基础实验教学中心|吉林长春 130012；3.吉林大学口腔医院牙周病科|吉林长春 130041

收稿日期:2008-11-04 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 佟巨慧;费瑞 E-mail:tongjuhui@sina.com;fayrui64@yahoo.com.cn
作者简介:付军权（1981-）|男|山东省烟台市人|理学硕士|主要从事多糖生物活性研究。
基金资助:
吉林省科技厅科技发展计划重点项目资助课题（20050413）；吉林省长春市科技计划项目资助课题（08QT10）

Therapeutic effect of silver carboxymethyl chitosan |on experimental periodontitis in rats

FU Jun-Quan¹, BI Rui¹, ZHANG Gui-Rong², CHI Li-Chao¹, WANG Shu-Xiang¹, TONG Ju-Hui³

1. Department of Cell Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China； 2. Experiment Center of Biology,School of Life Science,Jilin University,Changchun 130012,China；3. Department of Periodontics,Stomatology Hospital,Jilin University,Changchun 130041,China

Received:2008-11-04 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的：研究羧甲基壳聚糖银（CMCT-Ag⁺）对实验性大鼠牙周炎的治疗作用及机制，为研制具有高效杀菌作用的治疗牙周炎药物提供依据。方法：以钢丝结扎大鼠上颌右侧第一磨牙、涂牙龈卟啉菌和饲喂高糖软饲料方法建立实验性大鼠牙周炎模型。实验分正常组、模型组、替硝唑组和 12.5、50.0 及 200.0 mg·kg^-1 CMCT-Ag⁺组。测量各组大鼠的牙龈出血指数（BI）、牙菌斑指数（PLI）和牙槽骨吸收值（ABL）。结果：①与正常组比较，模型组牙龈易于出血，牙菌斑数增多，牙槽骨吸收明显；BI、PLI 和 ABL 均高于正常组（P<0.05 或 P<0.01）。② CMCT-Ag⁺治疗组与模型组比较：50.0 mg·kg^-1 组的 BI 和 PLI 及 200.0 mg·kg^-1 组 BI、PLI 和 ABL 显著低于模型组（P<0.05 或 P<0.01）。③ CMCT-Ag⁺ 治疗组与替硝唑组比较：12.5 mg·kg^-1 组的 BI 和 PLI 高于替硝唑组（P<0.01 ）。④ CMCT-Ag⁺治疗组：12.5 mg·kg^-1 组 BI 和 PLI 高于 200.0 mg·kg^-1 组（P<0.05）。结论：CMCT-Ag⁺对实验性大鼠牙周炎有明显治疗作用，并随浓度升高治疗作用增强，其中 200.0 mg·kg^-1 CMCT-Ag⁺对牙周炎大鼠的牙槽骨吸收有一定修复作用。CMCT-Ag⁺ 的作用机制主要是抑制牙周组织中的细菌，防止牙菌斑的形成。

关键词: 羧甲基壳聚糖银；牙周炎；牙龈卟啉菌

Abstract:

Abstract:Objective To investigate the therapeutic effect of silver carboxymethyl chitosan（CMCT-Ag⁺）on experimental periodontitis in rats and the therapeutic mechanism,and provide evidence for preparing drug of treatment for periodontitis with high bactericidal action.Methods The first molar on the right of upper jaw in rats was bound with steel-wire.The gums of rats were spread with Porphyromonas gingivalis and the rats were fed with food of high density of glucose to induce rat periodontitis model.The rats were divided into six groups:control,model,Tinidazole,12.5,50.0 and 200.0 mg·kg^-1 CMCT-Ag⁺.Then the bleeding index (BI),plaque index (PLI) and alveolar bone loss (ABL) in rats were observed. Results ① Compared with control rats,the model rats were easier gum blooding，dental plaque number increasing and conspicuous alveolar bone losing.The BI,PLI and ABL in model group were higher than those in control group（P<0.05 or P<0.01）.② Compared with model group,the BI and PLI in 50.0 mg·kg^-1 CMCT-Ag⁺group as well as BI,PLI and ABL in 200.0 mg·kg^-1 CMCT-Ag⁺group were lower than those in model group（P<0.05 or P<0.01）.③ Compared with Tinidazole group,the BI and PLI in 12.5 mg·kg^-1 CMCT-Ag⁺group were higher than those in Tinidazole group（P<0.01）.④ Compared between CMCT-Ag⁺ treatment groups,the BI and PLI in 12.5 mg·kg^-1 group were higher than those in 200.0 mg·kg^-1 group （P<0.05）.
Conclusion CMCT-Ag⁺ can effectively treat rat periodontitis in a dose-dependent manner and 200.0 mg·kg^-1 CMCT-Ag⁺can repair alveolar bone loss in periodontitis rats.The mechanism that CMCT-Ag⁺can treat rat periodontitis is due to inhibiting bacteria in periodontal tissue and preventing dental plaque from forming.

Key words: carboxymethyl chitosan-Ag⁺；periodontitis；Porphyromonas gingivalis

中图分类号:

R781.42

付军权, 费瑞, 张桂荣, 迟立超, 王书香, 佟巨慧. 羧甲基壳聚糖银对实验性大鼠牙周炎的治疗作用[J]. J4, 2009, 35(4): 669-672.

FU Jun-Quan, BI Rui, ZHANG Gui-Rong, CHI Li-Chao, WANG Shu-Xiang, TONG Ju-Hui. Therapeutic effect of silver carboxymethyl chitosan |on experimental periodontitis in rats[J]. J4, 2009, 35(4): 669-672.