关键词: 多重聚合酶链反应；大肠杆菌O157；副溶血性弧菌；普通变形杆菌；单核细胞增生李斯特菌

Abstract:

Abstract:Objective To  establish a rapid accurate method to detect four kinds of enteric pathogens including Listeria monocytogenes,Escherichia coli O157,Proteusbacillus vulgaris,Vibiro parahaemolyticus with multiplex PCR.Methods The special primers were designed of these bacteria for multiplex PCR respectively.Using this screening system to detect the commercially available juice which had been artificially randomly contaminated.Results Multiplex PCR could effectively amplify the corresponding gene fragments,the amlification fragments of Listeria monocytogene,Eschericha coli O157,Proteusbacillus vulgaris and Vibiro parahaemolyticus were 155,366,522 and 199 bp.The sensitivity of the pathogens  reached 103CFU/mＬ;as a result,any of three kinds of bacteria randomly contaminated in the juice could be identified successfully.Conclusion The use of multiplex PCR make it possible that whether the food is carrying the  four pathogenic bacteria mentioned above.

Key words: multiplex polymerase chain reaction;Escherichia coli O157;Proteusbacillus vulgaris;Vibiro parahaemolyticus;Listeria monocytogenes;