姜黄素及姜黄素衍生物A在大鼠血浆中含量的测定

J4 ›› 2012, Vol. 38 ›› Issue (3): 598-602.

姜黄素及姜黄素衍生物A在大鼠血浆中含量的测定

杨苹^1,隋思博²,王艳芳¹,杨晶¹,张敏^1,3,李校堃^1,3,4|郑琳⁵

1.吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春 130118；2.吉林省食品药品检验所|吉林长春 130033；3. 南京理工大学研究生院|江苏南京 210094；4.吉林大学白求恩医学院生物化学教研室|吉林长春 130021；5.辽宁省大连市环境监测中心|辽宁大连 116023

收稿日期:2011-11-22 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 张敏（Tel：0431-84533428，E-mail：372754079@qq.com）；李校堃（Tel：0431-84533348，E-mail：xiaokunli@163.net） E-mail:E-mail：372754079@qq.com；E-mail：xiaokunli@163.net
作者简介:杨苹（1987-）|女|山西省晋中市人|医学硕士|主要从事中药新药资源研究及开发应用的研究。
基金资助:
吉林农业大学青年基金资助课题(201127）；十一五“863”计划生物反应器重大专项基金资助课题（2007AA100503）

Determination of curcumin and curcumin derivative A in rat plasma

YANG Ping ¹,SUI Si-bo ²,WANG Yan-fang ¹,YANG Jing ¹,ZHANG Min ^1|3,LI Xiao-kun ^1,3,4,ZHENG Lin ⁵

1.Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education,Jilin Agricultural University,Changchun 130118,China；2. Jilin Institute for Food and Drug Control,Changchun 130033,China；3.Graduate |School,Nanjing University of Science and Technology,Nanjing 210094,China;4. Department of Biochemistry,Norman Bethune College of Medicine,Jilin University, Changchun 130021,China；5.Environmental Center of Dalian City,Dalian 116023,China

Received:2011-11-22 Online:2012-05-28 Published:2012-05-28

摘要/Abstract

摘要：

目的：建立大鼠血浆中姜黄素及姜黄素衍生物A的高效液相色谱(HPLC)测定方法，研究其在大鼠体内的药物动力学，为姜黄素的临床应用提供参考。方法：将姜黄素及姜黄素衍生物A分别灌胃给药，采用HPLC测定其在大鼠血浆中的血药浓度，色谱柱为Hypersil ODS2 C18（5 μm×4.6 mm×200 mm）；柱温：30℃；姜黄素流动相，乙腈-水-乙酸（体积比45∶55∶1），姜黄素检测波长为420 nm；姜黄素衍生物A流动相，乙腈-水-乙酸（体积比70∶30∶1）；体积流量为1.0 mL?min-1；姜黄素衍生物A检测波长为361 nm。结果：姜黄素及姜黄素衍生物A在0.25~100.00 mg?L-1 范围内与峰面积呈良好的线性关系，姜黄素回归方程为Y=105.90X－22.70，r=0.999 6；姜黄素衍生物A回归方程为Y=71.20X+24.62，r=0.999 4。两者日内、日间精密度RSD均小于4%，平均回收率均大于96%；大鼠灌胃给药后的药动学行为符合单室模型，姜黄素衍生物A的清除率比姜黄素显著降低，约是姜黄素清除率的3.57倍，曲线下面积是姜黄素的4.09倍，半衰期t1/2约为姜黄素的2.79倍。结论：HPLC测定姜黄素及姜黄素衍生物A在大鼠体内血药浓度的方法准确、简单可行、重复性好，为提高姜黄素在体内的稳定性，延长其在体内半衰期提供了依据。

关键词: 姜黄素；姜黄素衍生物；药物动力学；高效液

Abstract:

Objective
To develop the method of high performance liquid chromatography(HPLC) for determination of curcumin and curcumin derivative A in rat plasma and study their pharmacokinetics in rats, and to provide basis for clinical application of curcumin.Methods The curcumin and the curcumin derivative A were lavaged to the rats,and the concentrations of curcumin and curcumin derivative A in rat blood at different time points were determined by HPLC.The curcumin and curcumin derivative A were separated well on a Hypersil ODS2 C18 （5 μm×4.6 mm　×200 mm）under 30℃.The mobile phase of curcumin was a mixture of acetonitrile-water containing 1% acetic acid (45∶55) at a flow rate of 1 mL?min-1.The detective wavelength was 420 nm;the mobile phase of curcumin derivative A was a mixture of acetonitrile-water containing 1% acetic acid (70∶30) at a flow rate of 1 mL?min-1.The detective wavelength was 361 nm.Results The calibration curve was liner at the range of 0.25-100.00 mg?mL-1.The regression equation of curcumin was Y=105.90X－22.70，r=0.999 6；the regression equation of curcumin derivative A was Y=71.20X+24.62，r=0.999 4.The RSD of intra-day precision and inter-day precision was less than 4%.The average recovery was more than 96%.The plasma concentration-time curves of curcumin and curcumin derivative A in rats fit to one compartment model respectively.The clearance rate,the area under temperature and t1/2 of curcumin derivative A were 3.57,4.09 and 2.79 times respectively than those of curcumin.Conclusion The method of HPLC for the determination of curcumin and curcumin derivative A in plasma is accurate,simple and reliable,which provides the basis for the improvement of stablility and the extending of the half-time of curcumin.

Key words: curcumin；curcumin derivative；pharmacokinetics；high performance liquid chromatography

中图分类号:

R927.1

杨苹,隋思博,王艳芳,杨晶,张敏,3,李校堃|郑琳. 姜黄素及姜黄素衍生物A在大鼠血浆中含量的测定[J]. J4, 2012, 38(3): 598-602.

YANG Ping,SUI Si-bo,WANG Yan-fang,YANG Jing,ZHANG Min,LI Xiao-kun,. Determination of curcumin and curcumin derivative A in rat plasma[J]. J4, 2012, 38(3): 598-602.