关键词: 治疗应用, 转化生长因子-β_1<, sub>, 胶原重构, 大鼠, 近交SHR

Abstract: Objective To detect the effects of pancreatic kininogenase on the expression of TGF-β₁ and collagen remodeling in myocardiom of spontaneously hypertensive rats(SHR). Methods Twenty-four male SHR (aged fifteen weeks) were randomly divided into three groups: SHR group,pancreatic kininogenase group and captopril group（n=8），8 male Wistar Kyoto rats with normol blood pressure was considered as control group. Pancreatic kininogenase was given by peritoneal injection(7.2 U• kg^-1• d^-1), captopril was given by intragastric administration（10 mg• kg^-1• d^-1）, the rats in SHR group and control group were administered with 0.9% NaCl（2 mL • kg^-1• d^-1）through peritoneal injection.After four-week experiment, the pressure was measured in rats througth carotid artery ,the rats were sacrificed and left ventricular mass index, collagen volume fraction, peripheral vascular collagen area were measured.Myocardial tissue was stained with VG and pathological changes were observed. The expression of TGF-β₁ were detected by immunohistochemical technique(SP method). Results The systolic blood pressure, left ventricular mass index, collagen volume fraction, peripheral vascular collagen area and the expression level of TGF-β₁ in SHR group were obviously higher than those in control group (P<0.05). After treatment with pancreatic kininogenase for 4 weeks, all the indexes in pancreatic kininogenase group were obviously reduced compared with SHR group(P<0.05);but except systolic blood pressure,there were no significant differences of various indexes between pancreatic kininogenase and captopril groups(P>0.05).Conclusion Pancreatic kininogenase can obviously control pressure and reverse myocardial fibrosis probably by decreasing the expression of TGF-β₁ in SHR.

Key words: therapeutical use, TGF-β1, collagen remodeling, rats, inbred SHR