关键词: topiramate , 抗惊厥药, 脑水肿 , 细胞凋亡

Abstract: Objective To investigate the effects of topiramate on neurons apoptosis and the hydrous capacity in brain tissue in rats with perinatal acute ischemic brain damage. Methods The perinatal acute ischemic brain damage model was established by ligation of both uterine arteries of full-term pregnant Wistar rats(n=315). They were divided randomly into topiramate one and five times groups and hypoxic-ischemia (HI) group （n=105）, and normal collate group consisted of 105 normal Wistar rats .The effects of topiramate were observed by the changes of hippocampal apoptosis cells labeled in situ end TUNEL methods at different time spots (postnatal 3 h, 6 h, 24h, 3 d, 7 d, 14 d, 21 d, 28 d) and t he hydrous capacity in brain tissue within 7 d after birth.Results The hydrous capacity in brain tissue in administering drug one time group was remarkably less than that in HI group at 12 and 72 h after birth, and it in administering drug five time group was remarkably less than those in HI group and administering drug one time group at 48 and 72 h after birth（P<0.05）.The number of apoptosis neurons in administering drug one time group was remarkably less than those in HI group 24 h,3 d,7 d,21 d after birth, it in administering drug five time group was remarkably less than those in HI group and administering drug one time group 3 and 7 d after birth（P<0.05）. Conclusion Topiramate can remarkably lessen the degree of neurons apoptosis and shorten the duration of apoptosis; delay the occurrence of cerebral edema after HI, lessen the degree of cerebral edema, and shorten the duration of cerebral edema. The protective nerve function of topiramate in the many times administer ing drug group is obviously higher than that in the one time administering drug group.

Key words: topiramate , anticonvulsants , brain edema , apoptosis