溶血磷脂酸对豚鼠心室乳头肌动作电位及收缩力的影响

doi:吉林省科技厅自然科学基金资助课题（303705

溶血磷脂酸对豚鼠心室乳头肌动作电位及收缩力的影响

李欣，赵春燕，张文杰，赵明，姜川 

吉林大学基础医学院生理学教研室，吉林长春 130021

收稿日期:2006-04-28 修回日期:1900-01-01 出版日期:2007-01-28 发布日期:2007-01-28
通讯作者: 赵春燕

Effects of lysophosphatidic acid on action potential and contraction force in guinea pig papillary muscles

LI Xin, ZHAO Chun-yan, ZHANG Wen-jie, ZHAO Ming, JIANG Chuan

Department of Physiology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China

Received:2006-04-28 Revised:1900-01-01 Online:2007-01-28 Published:2007-01-28
Contact: ZHAO Chun-yan

摘要/Abstract

摘要： 目的：观察溶血磷脂酸（LPA）对离体豚鼠心室乳头肌动作电位及收缩力的影响。方法：健康成年豚鼠18只，随机分LPA 0.1、1.0、10.0 μmol•L^-1组，每组6只，采用给药前后自身对照的方法观察LPA对豚鼠乳头肌的作用。对10.0 μmol•L^-1组，灌流洗脱后，加入四乙基铵(TEA)20 mmol•L^-1+ LPA 10 .0 μmol•L^-1，观察TEA对LPA作用的影响。结果：LPA1.0和10.0 μmol•L^-1组乳头肌收缩幅度由给药前的100%增加到(122.6±7.9)% 和(139.48±8.2)%（P<0.05或P<0.01）。LPA 0.1 、1.0和10.0 μmol•L^-1组心室肌动作电位的幅度（APA）由给药前的（106.79±11.80 ）、（96.86±9.81）和（100.22±7.55）mV升高到（113.49±12.66）、（112.54±10.07）和（118.91±10.62）mV（P<0.05或P<0.01）；动作电位50%时程（APD₅₀）和动作电位90%时程(APD₉₀)均较给药前延长（ P<0.05）。20 mmol•L^-1的TEA 可使LPA 10.0 μmol•L^-1对APD₅₀的作用降低（P<0.05）。结论：LPA可增加豚鼠心脏乳头肌动作电位幅值，延长动作电位时程，增加心室肌收缩力。

关键词: 动作电位, 收缩力, 心律失常

Abstract: To observe the effects of lysophosphatidic acid（LPA）on the action potential and contraction force in guinea pig papillary muscles. Methods Eighteen healthy adult guinea-pigs were randomly divided into LPA 0.1,1.0, 10.0 μmol•L^-1groups, with six guinea pigs in every group compared with themselves before administration to observe the effects of LPA on the papillary muscles. The pigs in 10.0 μmol•L^-1 group were perfused with TEA 20 mmol•L^-1+ LPA 10 μmol•L^-1 after desorption. Results LPA 1.0,10.0 μmol•L^-1 could increase contraction force in guinea pigs compared with themselves before administration from 100% to (122.6±7.9)% and (139.48±8.2)%（P<0.05，P<0.01）. The LPA at different doses (0.1, 1.0, and 10.0 μmol•L^-1) could increase the action potential amplitude(APA) compared with themselves before administration from (106.79±11.80 ),(96.86±9.81),(100.22±7.55)mV to (113.49±12.66),(112.54±10.07),(118.91±10.62)mV（P<0.05 or P<0.01）；the action potential durations measured at the 50% repolarization levels and the 90% repolarization levels were prolonged compared with before administation（all P<0.05）.TEA(20 mmol•L^-1) shortened the APD₅₀ compared with LPA 10 μmol•L^-1 group（P<0.05）. Conclusion LPA could increase APA, prolong action potential duration and increase contractionforce .

Key words: action potential , contractile force , arrhythmia

中图分类号:

Q463

李欣，赵春燕，张文杰，赵明，姜川 . 溶血磷脂酸对豚鼠心室乳头肌动作电位及收缩力的影响[J]. J4, 2007, 33(1): 78-80.

LI Xin, ZHAO Chun-yan, ZHANG Wen-jie, ZHAO Ming, JIANG Chuan. Effects of lysophosphatidic acid on action potential and contraction force in guinea pig papillary muscles[J]. J4, 2007, 33(1): 78-80.

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