关键词: 糖尿病, 实验性, 肾脏, 结缔组织生长因子

Abstract: Objective To investigate the effect of benazepril on expression of connective tissue growth factor（CTGF）gene in kidney of diabetic rats and renal lesion. Methods Forty Wistar rats were randomly divided into control group(CON), diabetic group(DM) and diabetic rats treated with benazepril(DM+B) group. After benazepril was orally administered（15 mg·kg^-1· d^-1）to the diabetic rats for 12 weeks, serum creatinine (Scr) and blood urea nitrogen (BUN) levels, urinary protein excretion（UAER）and angiotensin Ⅱ (AngⅡ ) contents in renal cortex were determined. CTGF mRNA expression in the renal cortex was detected by RT-PCR. Immunohistochemstry was used to examine the expressions of fibronectin (FN) and type Ⅳ collagen (Col Ⅳ) in glomeruli. Results At the end of the experiment, UAER in DM group (12.92±3.56 mg·24 h^-1) was significantly higher than that in CON group (2.74 ±1.09 mg·24 h^-1)(P<0.01). The UAER in DM+B group (5.69±2.74 mg ·24 h^-1) was markedly lower than that in DM group (P<0.01). Diabetic rats showed an increase tendency in Scr (P<0.01) and BUN (P<0.01) contents compared with CON group, which were significantly reduced by benazepril treatment (P<0.01). CTGF mRNA expression in the renal cortex of DM group was 2.65 times that of CON group, and the expression of DM+B group was lower by 23.68% compared with that of DM group. The glomerular protein expressions of FN and Col IV were markedly up-regulated in DM group as compared with those in CON group (all P<0.01). Benazepril obviously decreased the expressions of FN and Col Ⅳ. Conclusion Benazepril reduces the increased CTGF mRNA expression in kidney of diabetic rats and ameliorates the renal lesions.

Key words: diabetes mellitus, experimental, kidney, connective tissue growth factor