关键词: 氟伐他汀, HL-60细胞, 诱导分化

Abstract:

Abstract:Objective To observe the effect  of fluvastatin (Flu) on differentiation and induction of human promyelocytic leukemia HL-60 cells and provide the  theoretical  foundation for treatment of human promyelocytic leukemia. Methods The cultured HL-60 cells were treated with  20 μmol·L^-1 Flu,the morphological changes  of the cell differentiation were examined.The NBT reduction capability was detected in HL-60 cells treated with Flu for 72 h.After HL-60 cells were treated with 20  μmol·L^-1 Flu for 5 d,  they were stained with non-specific esterase and the percentage of differentiation cells was analyzed.Results The HL-60 cells treated with Flu showed smaller cell body,reducing proportion of nucleus to cytoplasm,the nucleus tortuosity,fold or sublobe.There were specific granules and vacuoles in cytoplasm,displaying that some cells had differentiated to relative mature cells.As compared with control group,the NBT reduction capability in HL-60 cells treated with Flu for 72 h was significantly higher than that in control cells (P<0.01),which was similar to that of ATRA (10  μmol·L^-1.After HL-60 cells were treated with 20  μmol·L^-1Flu for 5 d,the percentages of positive cells were markedly higher than those in untreated HL-60 cells,and the positive reaction was not inhibited by NaF.Conclusion Flu treatment can result in differentiation of HL-60 cells to relative mature cells,it indicates that Flu may be used as a drug for treatment of human promyelocytic leukemia.

Key words: fluvastatin, HL-60 cell, induction of differentiation