关键词: 小核, 遗传学, 基因疗法, 嵌合体核酶, 聚合酶链反应

Abstract: Objective The U₁ small nuclear RNA(snRNA) has been exploited as a vector to construct U₁-chimeric ribozyme (U₁-Rz). Methods The DNA fragment of U₁-Rz was obtained by PCR on the pBSIISK+ U₁ containing the entire wide type human U₁ sequence. One of the primers was utilized containing the hammer head ribozyme gene sequence including its active core and blanking sequences, which targeted HCV core region. Twice subcloning were performed subsequently to construct the chimeric. Results The sequencing result showed that the third stem-loop of U₁ was just replaced by the ribozyme sequences. Conclusion U₁ snRNA chimeric ribozyme can be constructed by gene cloning strategy in vitro.

Key words: small nuclear, genetics, gene therapy, chimeric ribozyme, polymerase chain reaction