红参中精氨酸双糖苷对小鼠的抗疲劳作用

doi:10.13481/j.1671-587x.20170508

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (05): 897-902.doi: 10.13481/j.1671-587x.20170508

红参中精氨酸双糖苷对小鼠的抗疲劳作用

黄宝亮¹, 丁传波¹, 王佳奇¹, 郑毅男¹, 刘文丛¹, 张晶¹, 徐晓华²

1. 吉林农业大学中药材学院中药化学室, 吉林长春 130118;
2. 吉林大学中日联谊医院肾内科, 吉林长春 130033

收稿日期:2017-01-10 出版日期:2017-09-28 发布日期:2017-09-29
通讯作者: 张晶,教授,硕士研究生导师(Tel:0431-84533358,E-mail:zhjin0701@163.com);徐晓华,副主任医师,硕士研究生导师(Tel:0431-84995138,E-mail:xuxiaohua681002@qq.com) E-mail:zhjin0701@163.com;xuxiaohua681002@qq.com
作者简介:黄宝亮(1987-),男,内蒙古呼伦贝尔市人,医学硕士,主要从事天然产物研究与开发利用方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20150311050YY）；吉林省科技厅自然科学基金项目资助课题（20160101017JC）；吉林省教育厅"十三五"科学技术项目资助课题（吉教科合字[2015]第197号）

Anti-fatigue effect of arginylfructosylglucose from red ginseng in mice

HUANG Baoliang¹, DING Chuanbo¹, WANG Jiaqi¹, ZHENG Yinan¹, LIU Wencong¹, ZHANG Jing¹, XU Xiaohua²

1. Department of Traditional Chinese Medicine Chemistry, College of Traditional Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China;
2. Department of Nephrology, China-Japan Union Hospital, Jilin University, Changchun 130033, China

Received:2017-01-10 Online:2017-09-28 Published:2017-09-29

摘要/Abstract

摘要： 目的：观察小鼠强迫性游泳诱发的疲劳现象，探讨红参中非皂苷类物质——精氨酸双糖苷（AFG）对小鼠的抗疲劳作用及其初步机制。方法：从红参中分离出AFG提取物，将80只ICR小鼠分为空白对照组，低、中和高剂量（100、200和400 mg·kg^-1） AFG组，每组20只，连续灌胃给药28 d后对小鼠进行强迫性游泳实验，检测各组小鼠体质量，脏器指数，强迫性游泳时间，乳酸（LD）、尿素氮（BUN）、肝糖原（Gly）水平及腓肠肌中PGC-1α基因表达水平。结果：与空白对照组比较，低、中和高剂量AFG组小鼠的体质量和脏器指数差异无统计学意义（P > 0.05）；与空白对照组比较，低、中和高剂量AFG组小鼠强迫性游泳时间、Gly水平和PGC-1αmRNA基因表达水平均明显增加（P < 0.05或P < 0.01），且呈剂量依赖性；与空白对照组比较，低、中和高剂量AFG组小鼠血清中的LD和BUN水平明显降低（P < 0.01）。结论：AFG对小鼠有抗疲劳作用，其机制可能与能量代谢有关。

关键词: 精氨酸双糖苷, 力竭性游泳, 实时荧光定量聚合酶链反应, 免疫印迹法, 红参

Abstract: Objective: To investigate the anti-fatigue phenomenon induced by forced swimming in the mice,and to explore the anti-fatigue effect of argininyl fructoyl glucose(AFG)from red ginseng in the mice and its mechanism. Methods: The AFG was extracted from red ginseng. The ICR mice were divided into blank control group,low dose of AFG group(100 mg·kg^-1),middle dose of AFG group(200 mg·kg^-1) and high dose of AFG group(400 mg·kg^-1)(n=20). The mice mere given a forced swimming test after continuous gavage for 28 d. The weights,organ indexes,time of forced swimming,contents of lactic acid (LD),blood urea nitrogen (BUN),hepatic glycogen (Gly) and expressing levels of PGC-1α in gastrocnemius of the mice in various groups were detected. Results: Compared with blank control group,the weights and organ indexes of the mice in low,middle and high doses of AFG groups had no significant differences(P>0.05). Compared with blank control group,the time of forced swimming,contents of Gly and expressing levels of PGC-1α of the mice in low,middle and high doses of AFG groups were significantly increased(P<0.05 or P<0.01)in a dose-dependent manner. Compared with blank control group,The contents of LD and BUN in serum of the mice in low,middle and high doses of AFG groups were significantly decreased(P<0.01). Conclusion: AFG has anti-fatigue effect in mice,and its mechanism may be related to energy metabolism.

Key words: red ginseng, exhaustive swimming training, real-time fluorescence quantitative polymerase chain reaction, Western blotting method, arginyl fructosyl glucose

中图分类号:

R285

黄宝亮, 丁传波, 王佳奇, 郑毅男, 刘文丛, 张晶, 徐晓华. 红参中精氨酸双糖苷对小鼠的抗疲劳作用[J]. 吉林大学学报(医学版), 2017, 43(05): 897-902.

HUANG Baoliang, DING Chuanbo, WANG Jiaqi, ZHENG Yinan, LIU Wencong, ZHANG Jing, XU Xiaohua. Anti-fatigue effect of arginylfructosylglucose from red ginseng in mice[J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 897-902.

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红参中精氨酸双糖苷对小鼠的抗疲劳作用

Anti-fatigue effect of arginylfructosylglucose from red ginseng in mice

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