实验性哮喘小鼠T淋巴细胞对B淋巴细胞产生特异性IgG的调节作用

doi:国家自然科学基金资助课题(39570680)

实验性哮喘小鼠T淋巴细胞对B淋巴细胞产生特异性IgG的调节作用

胡素贤^1,2，黄瑾^2*

1. 福建医科大学附属厦门第一医院呼吸内科，福建厦门361003；2. 吉林大学第二医院呼吸内科，吉林长春130041

收稿日期:2004-08-25 修回日期:1900-01-01 出版日期:2005-09-28 发布日期:2005-09-28
通讯作者: 黄瑾

Regulation of T lymphocytes on antigen-specific IgG from B lymphocytes in experimental asthmatic mice

HU Shu-xian^1,2,HUANG Jin^2*

1. Department of Respiratory Medicine, Xiamen First Hospital Affiliated to Fujian Medical University, Xiamen 361003,China;2. Department of Respiratory Medicine,Second Hospital, Jilin University,Changchun 130041,China

Received:2004-08-25 Revised:1900-01-01 Online:2005-09-28 Published:2005-09-28
Contact: HUANG Jin

摘要/Abstract

摘要： 目的：探讨小鼠哮喘模型中T淋巴细胞对B淋巴细胞产生特异性IgG（sIgG）的调节作用。方法：雌性Balb/c小鼠40只，随机分成实验组（哮喘组）和对照组，每组各20只，实验组给予腹腔注射鸡卵白蛋白（OVA）6.7 mg·kg^-1（第2~4周）及雾化吸入1% OVA，20 min·d^-1（第5~6周），对照组注射同等剂量盐水及吸入无菌PBS。断头法处死小鼠，分离小鼠脾脏T、B淋巴细胞，分组进行细胞培养，以ELISA法检测各组细胞培养上清中sIgG的含量。结果：无论特异性抗原(OVA)是否参与，哮喘组小鼠B淋巴细胞sIgG分泌水平均明显高于对照组（P<0.05, P<0.01）；哮喘组小鼠T淋巴细胞能使其自身B淋巴细胞sIgG分泌水平明显下降（P<0.05）；抗原OVA刺激不能改变对照组和哮喘组小鼠B淋巴细胞自然状态下以及受刺激后的sIgG分泌水平（P＞0.05）；哮喘组小鼠T淋巴细胞能使对照组B淋巴细胞sIgG分泌水平升高(P＜0.05)；经哮喘组小鼠T淋巴细胞作用后，哮喘组鼠B淋巴细胞分泌sIgG水平低于对照组(P<0.05)；对照组小鼠T淋巴细胞能降低哮喘组小鼠B淋巴细胞sIgG分泌水平(P<0.05)。结论:在支气管哮喘中，B淋巴细胞sIgG参与了哮喘发病，T淋巴细胞对B淋巴细胞分泌sIgG具有显著调节作用，T淋巴细胞在支气管哮喘发病中起重要作用。

关键词: 免疫球蛋白G, T淋巴细胞, B淋巴细胞, 卵白蛋白

Abstract: Objective To study the regulation of T lymphocytes on antigen-specific IgG (sIgG) from B lymphocytes in asthmatic mouse models and clarify the role of T lymphocytes in the pathogenesis of asthma. Methods Firstly asthma mouse models were established. Then the mouse-ventilation-function-testing system was used to select mice which were successfully immunized. T and B lymphocytes were isolated from the mouse spleen aseptically and were cultivated in different combination. Then the supernate was collected and the sIgG in the supernate were tested with enzyme-linked-immunosorbent-assay (ELISA). Results No matter if the specific antigen was involved, sIgG secreted from B cells from asthmatic mice was higher than that of the B cells from control mice (P<0.05 and P<0.01). After regulated by its own T lymphocytes, levels of sIgG secreted by B cells from asthmatic mice decreased significantly (P<0.05). No matter if the T cells from asthmatic mice was involved, the specific antigen couldn′t change the levels of sIgG secreted by B cells from asthmatic and control mice (P>0.05); after interfered by T lymphocytes from asthmatic mice, levels of sIgG produced by B cells from normal control were increased obviously (P<0.05.); after regulated with T lymphocytes from asthmatic mice, levels of sIgG produced by B cells from asthmatic mice was lower than that of the B cells from control mice (P<0.05). With or without specific antigen, after regulated with T cells from asthmatic mice, levels of sIgG produced from B cells of asthmatic mice decreased obviously (P<0.05 and P<0.01). Conclusion sIgG is involved in the mechanism of asthma and T lymphocytes have a key role in regulating the pathogenesis of asthma. And T lymphocyte functional abnormality is one of the important mechanisms of asthma.

Key words: immunoglobulin G, T-lymphocyts, B-lymphocytes, ovalbumin

中图分类号:

R562.25

胡素贤，黄瑾. 实验性哮喘小鼠T淋巴细胞对B淋巴细胞产生特异性IgG的调节作用[J]. J4, 2005, 31(5): 671-674.

HU Shu-xian,HUANG Jin. Regulation of T lymphocytes on antigen-specific IgG from B lymphocytes in experimental asthmatic mice[J]. J4, 2005, 31(5): 671-674.

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