胰岛素样生长因子Ⅰ对人牙周膜成纤维细胞增殖的影响

doi:吉林省科技厅科研基金资助课题（20020616）

胰岛素样生长因子Ⅰ对人牙周膜成纤维细胞增殖的影响

罗云纲¹，刘晓秋²，朱孝民³，阎晓冬⁴

（1.吉林大学第二医院口腔科，吉林长春 130041；2. 吉林大学口腔医院修复科，吉林长春 130041；3. 吉林大学第二医院医务科，吉林长春 130041；4.吉林省长春市中心医院口腔科，吉林长春 130052）

收稿日期:2007-10-26 修回日期:1900-01-01 出版日期:2008-09-28 发布日期:2008-09-28
通讯作者: 罗云纲

Effect of insulin-like factor-[KG-*3]Ⅰ on proliferation activity of human periodontal ligament fibroblasts

LUO Yun-gang¹,LIU Xiao-qiu²,ZHU Xiao-min³,YAN Xiao-dong¹

(1.Department of Stomatology, Second Hospital, Jilin University, Changchun 130041,China;2.Department of Prosthodontics, Stomatology Hospital,Jilin University, Changchun 130041,China；3. Department of Medical Affairs，Second Hospital, Jilin University, Changchun 130041,China；4. Department of Stomatology, Changchun Central Hospital, Jilin Province,Changchun 130052,China)

Received:2007-10-26 Revised:1900-01-01 Online:2008-09-28 Published:2008-09-28
Contact: LUO Yun-gang

摘要/Abstract

摘要： 目的：研究胰岛素样生长因子Ⅰ（IGF-Ⅰ）对人牙周膜成纤维细胞( PDLF)增殖能力的影响，为牙周组织改建工作提供理论依据。方法：取本实验室自行制备生长状态良好的转染IGF-Ⅰ的PDLF和未转染IGF-Ⅰ的PDLF，分别用0.25%胰蛋白酶消化，制成细胞悬液，经计数后进行接种并计数，绘制成细胞生长曲线，比较2种细胞生长速度的差异；用0.25%胰蛋白酶分别消化转染和未转染IGF-Ⅰ的PDLF，培养后采用MTT法测定A值，并比较2种细胞增殖活性的差异;采用碱性磷酸酶（ALP）比色法检测比较转染和未转染IGF-Ⅰ的PDLF的ALP活性。结果：细胞生长曲线显示转染IGF-Ⅰ的PDLF的生长速度高于未转染IGF-Ⅰ的PDLF，差异有显著性（P<0.05）；转染IGF-Ⅰ的PDLF的增殖能力及ALP活性高于未转染IGF-Ⅰ的PDLF，差异有显著性（P<0.05或P<0.01）。结论： IGF-Ⅰ可能通过PDLF发挥其调节牙周组织改建的作用。

关键词: 牙周膜成纤维细胞, 转染

Abstract: To investigate the effect of insulin-like factor-I(IGF-Ⅰ)on the proliferation activity of human periodontal ligamental fibroblasts(PDLF).Methods The PDLF transfected with IGF-Ⅰ and PDLF untransfected with IGF-Ⅰ prepared in our lab were digested by 0.25% trypsin and prepared into cell suspension.The two sorts of cells were counted after being vaccinated, then the growth curves of the two cells were mapped and the growth speeds were compared.The PDLF transfected with IGF-Ⅰ and PDLF untransfected with IGF-Ⅰ were digested by 0.25% trypsin and then cultivated.The proliferation activities of the PDLF transfected with IGF-Ⅰ and PDLF untransfected with IGF-Ⅰ were observed using MTT methods.The activity of alkaline phosphatase(ALP) was determined by using ALP kit.Results The growth curves of the two cells showed that the growth speed of the PDLF transfected with IGF-Ⅰ was significantly faster than that of the PDLF untransfected with IGF-Ⅰ (P<0.05).The proliferation ability and ALP activity of the PDLF transfected with IGF-Ⅰ were both significantly higher than those of the PDLF untransfected with IGF-Ⅰ (P<0.05 or P<0.01). Conclusion IGF-Ⅰ may participate in the reconstruction of peridontal tissue through the function of PDLF.

Key words: periodontal ligament fibroblost, transfection

中图分类号:

R780.2

罗云纲，刘晓秋，朱孝民，阎晓冬. 胰岛素样生长因子Ⅰ对人牙周膜成纤维细胞增殖的影响[J]. J4, 2008, 34(5): 829-832.

LUO Yun-gang,LIU Xiao-qiu,ZHU Xiao-min,YAN Xiao-dong. Effect of insulin-like factor-[KG-*3]Ⅰ on proliferation activity of human periodontal ligament fibroblasts[J]. J4, 2008, 34(5): 829-832.

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