关键词:  胃肿瘤；维甲酸-干扰素诱导死亡相关基因-19； 转录信号传导子与激活子3；信号转导通路

Abstract:

Objective To study the expressions of GRIM-19 and its target gene product STAT3 in human gastric carcinoma（GC）tissues and their clinical  significances,and clarify their effects in occurrence and development of gastric carcinoma.  Methods  RT-PCR,immunohistochemistry dyeing and Western blotting were used to detect the mRNA and protein expressions of GRIM-19 and STAT3  in GC tissues，tissues surrounding carcinoma and normal gastric tisssues. The correlations between  the expressions of  GRIM-19 and STAT3 and  the  clinicopathologic characteristics of gastric carcinoma were analyzed statistically. Results The mRNA and protein levels of GRIM-19  in GC tissues as well as tissues surrounding carcinoma were obviously lower than those in normal tissues (P<0.01). Furthermore,the expression of GRIM-19 protein  was correlated to cell differentiation and clinical stage of GC (P<0.01). In contrast,the mRNA and protein levels of STAT3  as well as tissues surrounding carcinoma in GC tissues were higher  than those in normal tissues (P<0.01),and the expression of STAT3 was correlated to cell differentiation,lymphatic metastasis and clinical stage of GC(P<0.01). The significantly negative correlation was found between the expressions of GRIM-19 and STAT3 in GC tissues (r=－0.396，P<0.01). Conclusion
The low expression of GRIM-19 and the high expression of STAT3 co-exist in GC tissues. It may be related to malignant transformation and abnormal proliferation of cells,which facilitates the development of GC.

Key words: stomath neoplasm;gene associated with retiroid-interferon mortality-19;signal transducer and activator of transcription 3;signal transduction pathway