关键词: 药理学, 环磷酰胺, 淋巴细胞增殖

Abstract: Abstract:Objective To investigate the anti-mutation of Chinese medicinal herb Herba Leonuri and its effect on T lymphocyte proliferation in spleen.Methods The micronucleus test of mouse bone marrow cell (MNT) :thirty mice were divided into six groups (n= 5)，negative control ( NS)，cyclophosphamide group (CP 3.0 mg•kg^-1)，Herba Leonuri antimutagenesis groups(Herba Leonuri with dosages of 1.0,2.0,4.0,8.0 g•kg^-1+CP30 mg•kg^-1).The improved method was used to detect the micronuclei frequency.Lymphocyte transformation test: twenty-four mice were divided into four groups (n=6)，saline control，CP control (30 mg•kg^-1)，Herba Leonuri (2.0 g•kg^-1)，Herba Leonuri +CP (2.0 g•kg^-1 Herba Leonuri +CP 30 mg•kg^-1). MTT assay was used to calculate the stimulation index (SI).Results The micronuclei frequencies in Herba Leonur 2.0,4.0,8.0 g•kg^-1 groups were lower than that in CP group (P<0.05);the SI in saline control, Herba Leonuri，Herba Leonuri +CP,CP groups were 1.89±0.19, 2.17±0.14, 1.73±0.13 and 1.45±0.09,respectively, the difference of SI was significant between Herba Leonuri and saline control groups (P<0.05); the difference of SI was significant between Herba Leonuri +CP and CP groups(P<0.05).Conclusion Herba Leonuri may protect genetic material from injury caused by CP, namely,showing effective antimutagenesis and yet Herba Leonuri could enhance lymphocyte function.

Key words: pharmacology, cyclophosphamide, lymphocyte proliferation