大鼠骨髓间充质干细胞促肝组织损伤修复的作用

doi:吉林省卫生厅医学科研基金资助课题（200131

大鼠骨髓间充质干细胞促肝组织损伤修复的作用

高羽亭^1,2，陈强¹，丁国永¹，范东艳¹，范洪学¹

1.吉林大学公共卫生学院卫生毒理学教研室，吉林长春 130021；2.广东医学院公共卫生学院劳动卫生与环境卫生学教研室，广东东莞 523808

收稿日期:2007-11-28 修回日期:1900-01-01 出版日期:2008-09-28 发布日期:2008-09-28
通讯作者: 范洪学

Effect of bone marrow mesenchymal stem cells of rats on recovery of hepatic tissue injury

GAO Yu-ting^1,2, CHEN Qiang¹, DING Guo-yong¹, FAN Dong-yan¹, FAN Hong-xue¹

1.Department of Toxicology, School of Public Health, Jilin University,

Received:2007-11-28 Revised:1900-01-01 Online:2008-09-28 Published:2008-09-28
Contact: FAN Hong-xue

摘要/Abstract

摘要： 目的：探讨骨髓间充质干细胞（BMSCs）促肝组织缺血-再灌注损伤修复的作用。为肝脏疾病治疗提供新的思路。方法：①取健康Wistar周龄大鼠的骨髓细胞悬液，进行BMSCs体外扩增培养与分化潜能检测。②健康Wistar成年大鼠随机分为实验组（30只）和对照组（10只）。建立肝组织缺血-再灌注损伤模型，将BMSCs肝局部移植到实验组，对照组移植生理盐水，1周后观察两组肝缺血-再灌注损伤后大鼠的存活率以及肝脏修复情况，并通过蓝色荧光标记观察供体BMSCs在受体内的踪迹，探讨BMSCs促宿主损伤肝组织修复的机制。结果：①分离培养的BMSCs增殖旺盛，纯度较高，且均质性和稳定性好；用诱导剂诱导后，BMSCs具有向成骨细胞和脂肪细胞分化的能力。②实验组大鼠成活率达80%，肝细胞逐渐恢复正常，损伤肝组织得到修复，在宿主恢复的肝组织中发现了较多的带有蓝色荧光的细胞存在，而对照组（未移植BMSCs）大鼠腹腔积水、粘连，成活率达30%。2组成活率比较差异有显著性（P<0.05）。结论：BMSCs在体外易分离培养，具有多向分化的潜能；局部移植BMSCs后，可提高大鼠肝组织缺血-再灌注损伤后的恢复。

关键词: 肝组织, 局部移植

Abstract: Abstract:Objective To study the effect of bone marrow mesenchymal stem cells (BMSCs) promoting the recovery of injured hepatic tissue and provide a new method for the treatment of liver diseases. Methods ①BMSCs were cultivated and amplified in vitro from bone marrow cell suspension of rats.Then the potential multi-differentiation of BMSCs was detected.②Healthy Wistar rats were randomly divided into experimental group (n=30) and control group (n=10).The hepati ischemia-reperfusion injury model was established.BMSCs were transplanted into ischemia-reperfusion livers of rats in experimental group via local transplantation and saline was transplanted into the rats in control group.The survival rate of rats was observed, as well as the recovery of liver.The mechanism of the reparation of hepatic injury was explored through observation of the trace of DAPI-labeling donator’[KG-*3]s BMSCs in acceptor’[KG-*3]s body. Results ①BMSCs not only had a high purification, homogeneity and stability, but also had unlimited proliferation.BMSCs could differentiate into osteoblast and adipocyte by being cultivated in induced media of osteoblast differentiation and adipocyte differentiation.②The survival rate of experimental group was 80%. The injured hepatic tissues were repaired.The blue fluorescent cells were found in the recovery of the host liver tissue.The survival rate of control group (non BMSCs implantation) was up to 30%.The survival rates of the two groups had a significant difference (P<0.05). Conclusion BMSCs are easily isolated and cultivated in vitro.BMSCs can improve hepatic ischemia-reperfusion injury via local transplantation.

Key words: hepatic tissue, local transplantation

中图分类号:

R-332

高羽亭，陈强，丁国永，范东艳，范洪学. 大鼠骨髓间充质干细胞促肝组织损伤修复的作用[J]. J4, 2008, 34(5): 802-805.

GAO Yu-ting, CHEN Qiang, DING Guo-yong, FAN Dong-yan, FAN Hong-xue. Effect of bone marrow mesenchymal stem cells of rats on recovery of hepatic tissue injury[J]. J4, 2008, 34(5): 802-805.