三羟基苯甲酸纯化物及其化合物对Jurkat细胞周期进程及凋亡的影响

J4 ›› 2010, Vol. 36 ›› Issue (5): 904-907.

三羟基苯甲酸纯化物及其化合物对Jurkat细胞周期进程及凋亡的影响

吕喆¹, 牛凤兰², 郗艳丽², 龚守良¹

1. 吉林大学公共卫生学院卫生部放射生物学重点实验室|吉林长春 130021；2. 吉林大学公共卫生学院卫生检验教研室|吉林长春 130021

收稿日期:2010-03-30 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 牛凤兰（Tel:0431-85619133,E-mail:jluniu@163.com） E-mail:jluniu@163.com
作者简介:吕喆（1953-）|女|吉林省长春市人|高级工程师|主要从事电离辐射生物效应和肿瘤生物治疗的研究。
基金资助:
吉林省科技厅重点项目资助课题（20070424）

Effects of purified product of trihydroxybenzoic acid and its compound on cell cycle progression and apoptosis of Jurkat cells in vitro

LV Zhe¹, NIU Feng-Lan², XI Yan-Li², GONG Shou-Liang¹

Received:2010-03-30 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：研究不同浓度菱角三羟基苯甲酸（TBA）纯化物及其化合物对人 T淋巴细胞白血病Jurkat细胞周期进程及凋亡的影响，并探讨2种不同来源TBA对肿瘤生长抑制的可能机制。方法： 2种不同来源TBA分别作用于对数生长期的Jurkat 细胞，每种TBA分为5组（0、3.125、6.250、12.500和25.000 mg/L），作用于Jurkat 细胞30 h后，经流式细胞仪分别检测不同浓度菱角TBA纯化物及其化合物处理的Jurkat 细胞周期各时相及其凋亡率变化。结果： Jurkat细胞分别经过不同浓度菱角TBA纯化物处理后，细胞凋亡率明显高于0 mg/L组（P<0.05或P<0.01）；与对照组比较，3.125 mg/L组变化无差异；6.250、12.500和25.000 mg?L-1组变化有差异（P<0.05或P<0.01）。Jurkat细胞分别经过不同浓度TBA化合物处理后，与对照组比较，细胞凋亡率在3.125 mg?L-1组时即存在差异（P<0.05），随其剂量增加而增加（P<0.05 或P<0.01)，各用药组间变化无差异。经TBA纯化物作用后，G1期细胞百分数明显高于对照组（P<0.01），并随TBA剂量增加而增加，在6.250 ~ 25.000 mg/L浓度下S期细胞百分数明显低于对照组（P<0.05或P<0.01），G2期无显著变化。经TBA化合物作用后在6.250 ~ 25.000 mg/L浓度下G1期百分数明显高于对照组（P<0.05 或P<0.01），S期细胞百分数明显低于对照组（P<0.05 ），G2期无显著变化。各用药组间变化无差异。结论： 2种不同来源TBA均能够诱导细胞凋亡；均能够阻止人 T淋巴细胞白血病细胞的 G1期细胞向S期进程，其抑制作用可能通过G0/G1期阻滞引起。

关键词: 三羟基苯甲酸；Jurkat T细胞；细胞周期；细胞凋亡

Abstract:

Abstract:Objective To study the effects of purified product of trihydroxybenzoic acid (TBA) from water caltrop and its compound at different concentrations on cell cycle progression and apoptosis of Jurkat cells in vitro and explore their possible mechanism of inhibiting tumor growth. Methods The Jurkat cells at logarithmic growth phase were treated with purified product of TBA and its compound in vitro. Each TBA was divided into 5 groups (0,3.125,6.250,12.500 and 25.000 mg/L). The changes of cell cycle progression and apoptotic rate of Jurkat cells 30 h after treated with TBA were measured with flow cytometry. Results The apoptotic rates of the Jurkat cells treated with purified product TBA with doses of 3.125 and 25.000 mg/L were increased significantly as compared with those in control group(P<0.05 or P<0.01);the apoptotic rates of Jurkat cells treated with TAB compound with different doses were increased with the increase of doses (P<0.05 or P<0.01). The percentages of G1 phase cells treated with purified product TBA with different doses were increased significantly with the increase of doses as compared with those in control group(P<0.01),the percentages of S phase cells were decreased significantly in 6.250 and 25.000 mg/L groups（P<0.05 or P<0.01），and the percentages of G2 phase cells did not change significantly. The percentages of G1 phase cells treated with TAB compound with 6.250 and 25.000 mg/Lwere significantly higher than those in control group(P<0.05 or P<0.01),the percentages of S phase cells were decreased significantly (P<0.05),and the percentages of G2 phase cells did not change significantly. Conclusion Two different TBA could all induce Jurkat cell apoptosis and G1 phase arrest. Their inhibitory effects on tumor growth could be due to apoptosis caused by G1 arrest.

Key words: trihydroxybenzoic acid;Jurkat T cell;cell cycle;apoptosis

中图分类号:

R965

吕喆, 牛凤兰, 郗艳丽, 龚守良. 三羟基苯甲酸纯化物及其化合物对Jurkat细胞周期进程及凋亡的影响[J]. J4, 2010, 36(5): 904-907.

LV Zhe, NIU Feng-Lan, XI Yan-Li, GONG Shou-Liang. Effects of purified product of trihydroxybenzoic acid and its compound on cell cycle progression and apoptosis of Jurkat cells in vitro[J]. J4, 2010, 36(5): 904-907.