免疫组织化学染色在食管梭形细胞肿瘤诊断中的应用

J4 ›› 2010, Vol. 36 ›› Issue (5): 962-964.

免疫组织化学染色在食管梭形细胞肿瘤诊断中的应用

孙逊¹, 孟祥伟², 邹亚斌¹, 刘妍², 王新红³

1.吉林大学第一医院病理科|吉林长春 130021；2.吉林大学第一医院胃肠内科|吉林长春 130021；3.哈尔滨医科大学第二医院消化科|黑龙江哈尔滨150086

收稿日期:2010-07-01 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 孙逊(Tel:0431-88782630, E-mail:sunxun1961@yahoo.com.cn) E-mail:sunxun1961@yahoo.com.cn
作者简介:孙逊（1961-）|女|吉林省长春市人|副主任医师|主要从事消化道病理的研究。
基金资助:
吉林省科技厅科研基金资助课题（200505220）

Application of immunohistochemical staining in diagnosis of esophageal spindle cell tumor

SUN Xun¹, MENG Xiang-Wei², ZOU YA-Bin¹, LIU Yan², WANG Xin-Hong³

1.Department of Pathology,First Hospital,Jilin University,Changchun 130021,China ;2.Department of Gastroenterology|First Hospital,Jilin University,Changchun 130021,China;3.Department of Digestive Medicine, Second Hospital,Haerbin Medical University,Haerbin 150086, China

Received:2010-07-01 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：探讨免疫组织化学染色在食管梭形细胞肿瘤鉴别诊断及明确肿瘤的来源的意义。方法：应用HE染色及免疫组织化学染色法，检测18例食管梭形细胞肿瘤标本CD117、CD34、SMA、Desmin和S-100抗体的表达。结果：18例食管梭形细胞肿瘤中CD34、S-100、Desmin的阳性/阴性表达比例分别为4/14、0/18和12/6。CD117:6例阳性，其中2例局部胞膜阳性细胞，4例梭形细胞胞质阳性;12例阴性。SMA:17例肿瘤细胞呈程度不等的弥漫阳性。18例食管梭形细胞肿瘤中12例为平滑肌瘤，6例为间质瘤。结论: 免疫组织化学技术能比较准确地判断食管梭形细胞肿瘤的生物学行为和预后，为术后合理选择分子靶向治疗方案提供方向。

关键词: 免疫组织化学染色；食管肿瘤；梭形细胞

Abstract:

Abstract:Objective To investigate the significance of immunohistochemical method in differential diagnosis and definiting the origin of esophageal spindle cell tumors.Methods Eighteen cases of esophageal stromal turmors were studied by HE staning and immunohistochemistry to detect the expressions of CD117, CD34, SMA, Desmin and S-100.Results In 18 cases of esophageal spindle cell tumors,the CD34, S-100, Desmin positive/negative expression rates were 4/14,0/18 and 12/6 , respectively.6 cases of esophageal spindle cell tumors were positive for CD117,among them 2 cases were local positive in membrane and 4 cases were positive in cytoplasm of spindle cells. 12 cases of esophageal spindle cell tumors were negative for CD117. 17 cases tumor cells were varying degrees diffusely and positive expression for SMA. Among 18 cases of esophageal spindle cell tumors, 12 cases were leiomyoma and 6 cases were gastrointestinal stromal tumors. Conclusion Immunohistochemistry could accurately determine the biological behavior and prognosis of esophageal spindle cell tumors, it could provide direction for reasonable choice of molecular target therapy after operation.

Key words: immunohistochemical staining；esophageal neoplasms;spindle cell

中图分类号:

R735.1

孙逊, 孟祥伟, 邹亚斌, 刘妍, 王新红. 免疫组织化学染色在食管梭形细胞肿瘤诊断中的应用[J]. J4, 2010, 36(5): 962-964.

SUN Xun, MENG Xiang-Wei, ZOU YA-Bin, LIU Yan, WANG Xin-Hong. Application of immunohistochemical staining in diagnosis of esophageal spindle cell tumor[J]. J4, 2010, 36(5): 962-964.