关键词: 软骨细胞, 胶原, 纤维蛋白, 几丁质 R318.5,

Abstract: Objective To explore the method to construct articular cartilage with chitin-collagen scaffold. Methods Chondrocytes were isolated from articular cartilage of 2-week-old rabbits,digested by collagenase Ⅱ, and subcultured to passage 2 in vitro. After digested, the cells were mixed with bovine I type collagen, human blood freeze-dry fibrinogen, and thrombosin according to a certain proportion and mixed into a kind of net-like carrier that was made of chitin. After cultured one week in vitro, the culture was implanted into subcutaneous tissue in the back of a allogenic adult rabbit for 4 weeks. Results After one week, the cells appeared in triangle or like shuttle, and well distributed in the scaffold. The volume was no change obviously. After cultured in vivo,the chondrocyte secreted collagen Ⅱ and had invaded to the vessels and cells of inflammation. Conclusion Chondrocytes cultured in collagenⅠ,fibrin and chitin mixture may be used as a scaffold that can make tissue engineering cartilage in vitro or in vivo.

Key words: cartilage, chondrocyte, collagen, fibrin, chitin