重组人透明带蛋白3诱导精子的顶体反应

doi:国家自然科学基金资助课题

重组人透明带蛋白3诱导精子的顶体反应

王弘珺¹，张家颖²，左文静¹，许宗革¹，王忠山^1*，王野^△

1. 吉林大学基础医学院细胞生物学教研室，吉林长春130021；2. 吉林大学基础医学院生物化学与分子生物学实验中心，吉林长春130021

收稿日期:2002-12-21 修回日期:1900-01-01 出版日期:2004-07-28 发布日期:2004-07-28
通讯作者: 王忠山

Acrosome reaction of spermatozoa inducedby recombinant human zona pellucida 3

WANG Hong-jun¹, ZHANG Jia-ying², ZUO Wen-jing¹, XU Zong-ge¹, WANG Zhong-shan^1*,WANG Ye^△

1. Department of Cell Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021，China；2. Experimental Center of Biochemistry and Molecular Biology, Schoolof Basic Medical Sciences, Jilin University, Changchun 130021，China

Received:2002-12-21 Revised:1900-01-01 Online:2004-07-28 Published:2004-07-28
Contact: WANG Zhong-shan

摘要/Abstract

摘要： 目的：在体外条件下检测重组人透明带蛋白诱导的顶体反应。方法：体外采集已生育健康男子精液，采用非连续密度梯度法分离精子，将获能的精子悬液分成阴性对照组(C组，200 μL精子悬液加1 μL DMSO)、阳性对照组(A组，200 μL精子悬液加A23187至终浓度为10 μmol•L^-1)和ZP3组(200 μL精子悬液加ZP3至终浓度为10 mg•L^-1)，考马斯亮蓝染色法检测重组人ZP3蛋白诱导的顶体反应。结果：C组、A组和ZP3组发生顶体反应的精子百分率分别为(5.00±1.70)%、(46.10±7.49)%和(13.20±2.70)%，各组间比较差异有显著性(P<0.05)。结论：重组人透明带蛋白能够诱导人精子发生顶体反应。

关键词: 重组人透明带蛋白3, 精子

Abstract: Objective To detect the acrosome reaction induced by recombinant human zona pellucida 3 (rhZP3) in vitro. Methods The sperm from fertilized healthy men were obtained in vitro. The spermatozoa were isolated by discontinuous density gradient centrifugation, and the capacitated spermatozoa suspension were divided into negative control group (group C, 200 μL spermatoza suspension plus 1 μL DMSO), positive control group (group A, 200 μL spermatozoa plus A23187 to the end concentration of 10 μmol•L^-1) and ZP3 group (200 μL spermatozoa plus ZP3 to the end concentration of 10 mg•L^-1). The acrosome reaction induced by rhZP3 was detected with coomassie brilliant blue staining. Results The percentages of acrosome reaction of spermatozoa in group C, group A, and ZP3 group were (5.00±1.70)%,(46.10±7.49)%, and (13.20±2.70)%，respectively. There were significant differences between various groups (P<0.05).Conclusion The rhZP3 can induce acrosome reaction of spermatozoa.

Key words: recombinant human zona pellucida 3, spermatoza

中图分类号:

R321.1

王弘珺，张家颖，左文静，许宗革，王忠山，王野. 重组人透明带蛋白3诱导精子的顶体反应[J]. J4, 2004, 30(4): 512-514.

WANG Hong-jun, ZHANG Jia-ying, ZUO Wen-jing, XU Zong-ge, WANG Zhong-shan,WANG Ye. Acrosome reaction of spermatozoa inducedby recombinant human zona pellucida 3[J]. J4, 2004, 30(4): 512-514.

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