关键词: 免疫球蛋白E, T淋巴细胞, B淋巴细胞, 疾病模型, 动物

Abstract: Objective To study the regulation and interference of T lymphocyte on antigen-specific IgE (SIgE) antibodies by B lymphocyte in bronchial asthma mouse model and clarify the role of T lymphocyte in the pathogenesis of asthma. Methods The asthma mouse model was set up at first, then T and B lymhphocytes were isolated from the mice spleen and were cultivated in different combinations. The supernates were collected and the specific anti-OVA antibody IgE in the supernate were tested with enzyme linked immunosorbent assay (ELISA). Results The level of SIgE secreted by B cells from asthma mouse was higher than that from the control mouse (P<0.05). After regulated by own T lymphocytes, the level of SIgE secreted by B cells from asthma mouse decreased significantly (P<0.05). With or without specific antigen, after regulated by T cells from asthma mouse, the level of SIgE secreted by B cells from the control mouse increased, but there was no significant difference (P>0.05). Conclusion T lymphocyte has a key role in regulating and controlling the pathogenesis of asthma. And T cell functional abnormality is one of the important mechanisms of asthma.

Key words: immunoglobulin E, T-lymphocytes, B-lymphocytes, disease models, animal