关键词: 药物作用, 铅, 毒性, 精子, 畸形, 抗诱变药

Abstract: Objective To examine the inhibitory effects of Glycyrrhiza uralensis on sperm abnormalities induced by lead acetate,Pb(CH₃COO)₂, in mice. Methods Sixty-five mice were randomly divided into groups: negative control （N•S）, positive control（MMC 1.0 mg•kg^-1）， lead acetate mutagenesis groups（40，20, and 10 mg•kg^-1，which were matched 1/3，1/6, and 1/12 LD₅₀），Glycyrrhizia uralensis mutagenesis groups （1.25，2.50，5.00， and 10.00 g•kg^-1，which were matched 5×，10×，20×， and 40× clinical commonly used dosage of a person），Glycyrrhizia uralensis antimutagenesis groups （lead acetate 20 mg•kg^-1+ each Glycyrrhizia uralensis dosage groups）,5 mice per group. MMC and lead acetate were given by abdominal cavity injection, other drugs were given by perfusing stomach. Glycyrrhizia uralensis and lead acetate were given simultaneously in antimutagenesis groups,once a day for 5 days successively. At the 5th weekend after the first administration,the mice were killed by cervical vertebra dislocation whose epididymidis were drawed and sheets were produced in Wyrobek method.Results Sperm abnormality frequency (%) was no significant difference between tested groups（1.20±0.22，1.38±0.27，1.52±0.25，and 1.32±0.30）and control group（1.28±0.18） (P>0.05), but the frequency of abnormal sperms induced by lead acetate (%)（5.12±0.38） was reduced significantly by intragastrial perfusion of Glycyrrhiza uralensis and lead acetate （3.28±0.24，3.06±0.29，2.32±0.31， and 1.84±0.27）at the same time (P<0.01). Conclusion Glycyrrhiza uralensis can′t induce the sperm abnormality of mice,but shows a protective effect, namely antimutagenesis, on genetic materials in mice.

Key words: drug effects, lead, toxicity, spermatozoa, abnormalities, antimutagenic agents