痛风宁胶囊对小鼠高尿酸血症和肾脏功能的改善作用

doi:10.13481/j.1671-587x.20170507

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (05): 893-896.doi: 10.13481/j.1671-587x.20170507

痛风宁胶囊对小鼠高尿酸血症和肾脏功能的改善作用

樊海瑞¹, 赵岩¹, 傅警龙², 张海波², 王德超², 郜玉钢¹, 张连学¹

1. 吉林农业大学中药材学院人参工程技术研究中心, 吉林长春 130118;
2. 长中风湿骨病医院风湿科, 吉林长春 130000

收稿日期:2017-04-08 出版日期:2017-09-28 发布日期:2017-09-29
通讯作者: 赵岩,教授,硕士研究生导师(E-mail:zhyjlu79@163.com);张海波,副主任医师(E-mail:fengshiyiyuan@163.com) E-mail:zhyjlu79@163.com;fengshiyiyuan@163.com
作者简介:樊海瑞(1990-),女,山东省济宁市人,在读医学硕士,主要从事天然药物化学成分和生物活性方面的研究。
基金资助:
国家农业部公益性行业科研专项资助课题（201303111）；吉林省科技厅科技发展计划项目资助课题（20150204049YY，20140204013YY，20140311050YY）

Improvement effects of Tongfengning Capsule on hyperuricemia and renal function in mice

FAN Hairui¹, ZHAO Yan¹, FU Jinglong², ZHANG Haibo², WANG Dechao², GAO Yugang¹, ZHANG Lianxue¹

1. Research Center of Ginseng Engineering Technology, College of Chinese Medicine Material, Jilin Agricultural University, Changchun 130118, China;
2. Department of Rheumatism, Changzhong Rheumatism Hospital, Changchun 130000, China

Received:2017-04-08 Online:2017-09-28 Published:2017-09-29

摘要/Abstract

摘要： 目的：探讨痛风宁胶囊（TFN）对高尿酸血症模型小鼠血清中尿酸（UC）、肌酐（Cr）和尿素氮（BUN）水平以及小鼠肝脏匀浆液中黄嘌呤氧化酶（XOD）和腺苷脱氨酶（ADA）活性的影响，观察TFN对高尿酸小鼠肾脏组织病理学变化的改善作用，阐明其作用机制。方法：采用酵母膏合并氧嗪酸钾盐诱导建立小鼠高尿酸血症模型。将70只小鼠分为空白对照组，模型组，低（200 mg·kg^-1）、中（400 mg·kg^-1）和高剂量（800 mg·kg^-1） TFN组，别嘌醇（50 mg·kg^-1）和痛风舒（TFS，600 mg·kg^-1）阳性药对照组，每组10只，灌胃给药。实验结束后检测小鼠血清中UC、Cr和BUN水平以及小鼠肝脏匀浆液中XOD和ADA活性，HE染色观察小鼠肾脏组织病理表现。结果：与空白对照组比较，模型组小鼠血清中UC、Cr和BUN水平明显升高（P < 0.01），肝组织中XOD和ADA活性亦升高（P < 0.01）；与模型组比较，阳性药对照组，低、中和高剂量TFN组小鼠血清中UC、Cr和BUN水平明显降低（P < 0.01），肝组织中XOD和ADA活性降低（P < 0.05或P < 0.01），其中高剂量TFN组效果最为明显。光镜下观察，与模型组比较，阳性药对照组和高剂量TFN组小鼠肾脏肾小球萎缩、肾间质纤维化和肾小管扩张等病理变化得到一定程度上改善。结论：TFN对小鼠高尿酸血症具有一定的改善作用，其机制与抑制UC生成和促进UC排泄有关。

关键词: 痛风宁胶囊, 高尿酸血症, 酵母膏, 氧嗪酸钾盐

Abstract: Objective: To discuss the influence of Tongfengning Capsule(TFN)in the levels of uricacid(UC),creatinine (Cr) and urea nitrogen(BUN) in mouse serum and the activities of the xanthine oxidase(XOD),adenosine deaminase (ADA) activity in the liver homogenate of the mice with hyperuricemia, and to observe the improvement effect of TFN on the pathological changes of liver tissue and to clarify its mechanisms. Methods: The models of mouse hyperuricemia were induced by yeast extract with potassium oxonate. Seventy mice were divided into blank control group,model group, low(200 mg·kg^-1), medium(400 mg·kg^-1)and high(800 mg·kg^-1) doses of TFN groups, allopurinol positive drug control group(50 mg·kg^-1),Tongfengshu(TFS,600 mg·kg^-1) positive drug control group(n=10). The levels of UC,Cr,BUN in serum and the activities of XOD, ADA in homoggenate were detected and the histopathological changes of the kidney tissue of the mice were measured with HE staining. Results: Compared with blank control group, the levels of serum UC, Cr and BUN of the mice in model group were significantly increased(P<0.01),and the activities of XOD and ADA in liver tissue were also increased(P<0.01). Compared with model group, the levels of serum UC, Cr and BUN of the mice in positive drug control groups and different doses of TFN groups were decreased (P<0.01),and the activities of XOD and ADA in liver tissue were also decreased (P<0.05),especially in high dose of TFN group.Compared with model group,the pathologic changes such as renal glomerulus atrophy, renal interstitial fibrosis and expansion of renal tubule of the mice in positive drug control groups and high dose of TFN group were improved to a certain extent. Conclusion: TFN has improvement effcet on the hyperuricemia in the mice and its mechanism is related to the inhibition of uricogenesis and the promotion of UC excretion.

Key words: Tongfengning Capsule, hyperuricemia, yeast extract, potassium oxonate

中图分类号:

R589.7

樊海瑞, 赵岩, 傅警龙, 张海波, 王德超, 郜玉钢, 张连学. 痛风宁胶囊对小鼠高尿酸血症和肾脏功能的改善作用[J]. 吉林大学学报(医学版), 2017, 43(05): 893-896.

FAN Hairui, ZHAO Yan, FU Jinglong, ZHANG Haibo, WANG Dechao, GAO Yugang, ZHANG Lianxue. Improvement effects of Tongfengning Capsule on hyperuricemia and renal function in mice[J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 893-896.

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痛风宁胶囊对小鼠高尿酸血症和肾脏功能的改善作用

Improvement effects of Tongfengning Capsule on hyperuricemia and renal function in mice

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