J4 ›› 2010, Vol. 36 ›› Issue (3): 518-522.

• 基础研究 • 上一篇    下一篇

不同物理和化学因素对重组人角质细胞生长因子2稳定性的影响

 周鑫1,2, 王晓杰1,3,4, 田海山1,4, 唐禄1,2, 焦悦1, 朱家楠1, 王莘2, 李校堃1   

  1. 1.吉林农业大学 生物反应器与药物开发教育部工程研究中心,吉林 长春 130118;2.吉林
    农业大学生命科学学院,吉林 长春 130118;3.吉林大学公共卫生学院 卫生部放射生物学重点实验室,吉林 长春 130021;4.吉林省长春市格鲁斯特生物科技有限公司|吉林 长春 130012)
  • 收稿日期:2010-01-05 出版日期:2010-05-28 发布日期:2010-05-28
  • 通讯作者: 李校堃(Tel:0431-84533348,E-mail:xiaokunli@163.net);王 莘(Tel:0431-84533428,E-mail:wangxin5853@126.com) E-mail:xiaokunli@163.net
  • 作者简介:周 鑫(1984-)|女|吉林省吉林市人|在读理学硕士|主要从事基因工程制药方面的研究。
  • 基金资助:

    科技部“重大新药创制”科技重大专项基金资助课题(2008ZX09101-057) ;长春市应用技术研究计划项目资助课题(2008262);长春市企业科技特派员行动计划项目资助课题(09KT03);长春市科技型中小企业技术创新基金资助课题(09C26212203306)

Effects of different physical and chemical factors on stability of recombinant human keratinocyte growth factor-2

ZHOU Xin1,2, WANG Xiao-Jie1,3,4, TIAN Hai-Shan1,4, TANG Lu1,2, JIAO Yue1, ZHU Jia-Nan1, WANG Xin2, LI Jiao-Kun1   

  1. 1.Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education,Jilin Agriculture University,Changchun 130118,China;2.School of Life Science,Jilin Agriculture University,Changchun 130118,China;3.Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China;4.Changchun Grostre Biotechnology Co.,Ltd., Changchun 130012,China)
  • Received:2010-01-05 Online:2010-05-28 Published:2010-05-28

摘要:

目的:研究重组人角质细胞生长因子2 (rhKGF-2)在不同保存条件下的稳定性,阐明温度、pH值、缓冲溶液及反复冻融因素对rhKGF-2稳定性的影响作用。方法: rhKGF-2在不同温度(-20℃、4℃、25℃、40℃)、pH值(pH 4~9)、缓冲溶液(柠檬酸钠-柠檬酸缓冲液、磷酸氢二钠-柠檬酸缓冲液、磷酸盐缓冲液、吉斐氏缓冲溶液)及反复冻融条件下分别保存,采用RP-HPLC法检测rhKGF-2纯度,同时观察外观性状。结果:-20℃、4℃分别保存时,rhKGF-2的各项性质在观察期内都无明显变化;25℃时,rhKGF-2存放28 d纯度较0 h(100.00%)下降至(68.20±0.14)%;40℃时,rhKGF-2存放9 h纯度较0 h(100.00%)下降至(4.80±0.39)%。rhKGF-2(pH 6.0)在25℃条件下保存90 d纯度较0 h(100.00%)下降至(79.20±0.12)%,与对照组(0 h)比较差异有显著性(P<0.05);pH值为4.0、9.0的rhKGF-2液体在25℃条件下分别保存120 h纯度较0 h(100.00%)下降至(15.60±0.30)%和(67.30±0.10)%,与对照组(0 h)比较差异有显著性(P<0.01)。rhKGF-2在柠檬酸缓冲液中25℃条件下保存60 d纯度较0 h(100.00%)下降至(79.20±0.15)%,与对照组(0 h)比较差异有显著性(P<0.01)。rhKGF-2原液反复冻融后其纯度变化差异无显著性(P>0.05)。结论:rhKGF-2在低温和pH 6.0~7.0中性条件下稳定。高温、偏酸性和偏碱性条件下均不利于rhKGF-2稳定。柠檬酸缓冲液有利于rhKGF-2稳定。反复冻融对rhKGF-2稳定性影响不明显。

关键词: 重组人角质细胞生长因子-2;纯度;稳定性

Abstract:

To study the stabilities of recombinant human keratinocytegrowth factor-2(rhKGF-2) deposited at different conditions,and elucidate the influences of temperature,pH,buffer solutions and repeated freeze thawing on rhKGF-2 stability.Methods rhKGF-2 was deposited at different conditions such as temperatures (-20℃,4℃,25℃,40℃),pH(pH 4-9),buffer solutions(Citrate buffer solution,Disodium hydrogen phosphate-Citrate buffer,Phosphate buffer,Jifeishi buffer) and repeated freeze thawing, respectively.The purity of rhKGF-2 was determined by RP-HPLC,and the appearance was also observed.Results All properties of rhKGF-2 were not altered during the observation period at -20℃ and 4℃;the purity of rhKGF-2  deposied for 28 d was reduced to (68.20±0.14)% compared with 0 h(100.00%)  at 25℃;the purity of rhKGF-2  deposited for 9 h was reduced to (4.80±0.39)% compared with 0 h(100.00%)  at 40℃. The purity of rhKGF-2(pH 6.0) deposited for 90 d was  reduced to(79.20±0.12)% compared with 0 h(100.00%) at 25℃(P<0.05);the purities of rhKGF-2(pH 4.0,9.0) deposited for 120 h were respectively reduced to (15.60±0.3)% and (67.30±0.1)% compared with 0 h(100.00%)at 25℃(P<0.01).The purity of rhKGF-2(pH6.0) deposited in Citrate  buffer solution deposited for 60 d  was  reduced to (79.20±0.15)% compared with 0 h(100.00%)  at 25℃(P<0.01).The purity of rhKGF-2 had no significant change  after repeated freeze thawing(P>0.05).Conclusion rhKGF-2 is stable when deposited at low temperature and pH6.0-7.0 neutral conditions.High temperature,acidic and basic conditions are harmful for rhKGF-2 stability.It is profit for rhKGF-2 stability in Citrate buffer solution.There is no impact on rhKGF-2 stability after repeated freeze thawing.

Key words: recombinant human keratinocyte growth factor-2;purity;stability

中图分类号: 

  • Q5