卵巢浆液性囊腺癌组织中β-连环蛋白的表达及其意义

J4 ›› 2010, Vol. 36 ›› Issue (3): 536-539.

卵巢浆液性囊腺癌组织中β-连环蛋白的表达及其意义

张晓霞¹, 孙晓琦², 张松灵¹, 高咏梅¹, 田海涵³

(1.吉林大学第一医院妇产科|吉林长春 130021；2.福建省福州市妇幼保健院|福建福州 350000 ；3.内蒙古自治区通辽市红星医院|内蒙古通辽 028000)

收稿日期:2009-12-09 出版日期:2010-05-28 发布日期:2010-05-28
通讯作者: 张松灵 E-mail:slzhangsl@yahoo.com.cn
作者简介:张晓霞（1962-）|女|吉林省长春市人|副教授|主任医师|医学博士| 主要从事卵巢癌基因诊断与治疗的研究。
基金资助:
吉林省教育厅科研基金资助课题(吉教科合字［2005］132号)

Expression of β-catenin in ovarian serous cystadenocarcinoma tissues and its significances

ZHANG Xiao-Xia¹, SUN Xiao-Qi², ZHANG Song-Ling¹, GAO Yong-Mei¹, TIAN Hai-Han³

(1. |Department of Obstetrics and Gynecology,First Hospital,Jilin University,Changchun 130021,China;2. |Fuzhou Materinity and Child Health Hospital,Fuzhou 350000,China|3. |HongXing Hospital,Tongliao,Inner Mongolia Autonomous Region,Tongliao 028000,China )

Received:2009-12-09 Online:2010-05-28 Published:2010-05-28

摘要/Abstract

摘要：

目的：研究卵巢浆液性囊腺癌组织中β-连环蛋白（β-catenin）的表达，阐明β-连环蛋白在卵巢浆液性囊腺癌诊断中的临床意义。方法：采用RT-PCR法检测36例卵巢浆液性囊腺癌、10例卵巢浆液性囊腺瘤及10例正常卵巢组织β-连环蛋白mRNA的表达量。应用免疫组织化学方法检测β-连环蛋白在60例卵巢浆液性囊腺癌、24例卵巢浆液性囊腺瘤及24例正常卵巢组织中的表达率。结果：β-连环蛋白mRNA在卵巢浆液性囊腺癌组表达量为1.001±0.192，在正常卵巢组为0.588±0.128，在卵巢浆液性囊腺瘤组为0.600±0.262，卵巢浆液性囊腺癌组β-连环蛋白mRNA的表达量显著高于正常卵巢组和卵巢浆液性囊腺瘤组，差异有显著性（P<0.05)。β-连环蛋白主要在细胞质中表达，在卵巢浆液性囊腺癌组的β-连环蛋白阳性表达率为88.33%，明显高于正常卵巢组的20.83%和卵巢浆液性囊腺瘤组的33.33%（P<0.05)；在卵巢癌组临床分期Ⅰ的卵巢癌β-连环蛋白阳性表达率为77.77%，临床分期Ⅳ期的卵巢癌β-连环蛋白阳性表达率为93.87%，两者比较差异有显著性（P< 0.05）；在卵巢癌病理分级G1、G2及G3组的β-连环蛋白阳性表达率分别为85.00%、89.28%和91.63%，组间比较差异无显著性（P>0.05）。结论：卵巢癌组织中β-连环蛋白mRNA和蛋白表达均增高，β-连环蛋白表达程度与卵巢癌临床分期相关，与病理分级无关，β-连环蛋白在卵巢癌诊断中起重要作用。

关键词: 卵巢浆液性囊腺癌；β-连环蛋白；逆转录聚合酶链反应；免疫组织化学

Abstract:

To study the expression of β-catenin in ovarian serous cystadenocarcinoma tissues and disscuss the clinical significances of β-catenin in the diagnosis of ovarian serous cystadenocarcinoma.Methods RT-PCR method was used to detect the expressions of β-catenin mRNA in tissue samples of 36 ovarian serous cystadenocarcinoma,10 ovarian serous cystadenoma and 10 normal ovary tissues.The expressions of β-catenin in 60 ovarian serous cystadenocarcinoma,24 ovarian serous cystadenoma and 24 normol ovarian tissues were detected by immunohistochemistry.Results The expression level of β-catenin mRNA in ovarian serous cystadenocarcinoma tissues（1.001±0.192）was significantly higher than those in normal ovarian tissues（0.588±0.128）and ovarian serous cystadenoma tissues（0.600±0.262）（P＜0.05）.The expression of β-catenin was mainly located in cytoplasm;the positive rate of β-catenin in ovarian serous cystadenocarcinoma group was 88.33%,which was significantly higher than those in normal ovarian group(20.83%)and ovarian serous cystadenoma group(33.33%) (P<0.05).There was significant difference in the expression level of β-catenin in ovarian cancer between different clinical stages,while the positive rate in stage Ⅰ was 77.77% and in stage Ⅳ was 93.87%(P<0.05);however,there was no significant difference in the expression level of β-catenin in ovarian cancer between different pathlogical grades,while the positive rates in ovarian cancer for G1,G2 and G3 were 85.00%,89.28%,and 91.63%， respectively;there was no significant difference between various groups(P>0.05).Conclusion The expression level of β-catenin in ovarian cancer is higher,the expression level of β-catenin is associated with the clinical staging of ovarian cancer but unrelated with pathological grade;β-catenin may play an important role in the detection and diagnosis of ovarian cancer.

Key words: ovarian serous cystadenocarcinoma;β-catenin;reverse transcriptase polymerase chain reaction;immunohistochemistry

中图分类号:

R737.31

张晓霞, 孙晓琦, 张松灵, 高咏梅, 田海涵. 卵巢浆液性囊腺癌组织中β-连环蛋白的表达及其意义[J]. J4, 2010, 36(3): 536-539.

ZHANG Xiao-Xia, SUN Xiao-Qi, ZHANG Song-Ling, GAO Yong-Mei, TIAN Hai-Han. Expression of β-catenin in ovarian serous cystadenocarcinoma tissues and its significances[J]. J4, 2010, 36(3): 536-539.

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