促黑素促进骨骼肌细胞脂肪酸氧化中cAMP的作用

J4 ›› 2010, Vol. 36 ›› Issue (6): 1043-1046.

促黑素促进骨骼肌细胞脂肪酸氧化中cAMP的作用

金勇君¹, 杨美子², 张凌云¹, 王秀云¹, 邢绍芝¹

（1.滨州医学院附属医院内分泌科|山东滨州256603；2.滨州医学院药理学教研室|山东烟台 264003）

收稿日期:2010-04-27 出版日期:2010-11-28 发布日期:2010-11-28
作者简介:金勇君（1972-）|男|吉林省延吉市人|副教授|医学博士|主要从事肥胖和骨质疏松症的研究。
基金资助:
国家自然科学基金资助课题（30660070）;山东省教育厅科技计划项目资助课题（J07YD21）

Effect of cAMP on fatty acid oxidation in skeletal muscle cells by α-MSH

JIN Yong-Jun¹, YANG Mei-Zi², ZHANG Ling-Yun¹, WANG Xiu-Yun¹, XING Shao-Zhi¹

(1.Department of Endocrinology|Affiliated Hospital of Binzhou Medical University|Binzhou 256603|China；2. Department of Pharmacology|Binzhou Medical University|Yantai 264003|China）

Received:2010-04-27 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的：探讨骨胳肌细胞中环磷酸腺苷（cAMP）对脂肪酸氧化的影响，阐明促黑素（α-MSH）对脂肪酸氧化影响的作用机制。方法：采用α-MSH、cAMP类似物Bt2cAMP、8-BrcAMP和cAMP拮抗剂分别处理体外培养的原代骨骼肌细胞(PMC)和C2C12成肌细胞，分为α-MSH组、cAMP类似物组、cAMP拮抗剂加α-MSH组，同时设正常对照组，利用（9，10-3H）棕榈酸生成3H2O的方法测定脂肪酸氧化。RT-PCR法测定PMC和C2C12成肌细胞黑皮质素受体（MCR）的表达。结果：α-MSH组和cAMP类似物组与对照组比较脂肪酸氧化显著增加（P<0.05）；cAMP拮抗剂RpcAMP预处理组细胞脂肪酸氧化率明显降低，与对照组比较差异无显著性（P>0.05），与α-MSH组比较差异有显著性（P<0.05）。结论： α-MSH和cAMP类似物显著增加了脂肪酸氧化，α-MSH可能与MCR结合通过cAMP信号传导通路促进骨骼肌细胞的脂肪酸氧化。

关键词: 环磷酸腺苷；促黑素；脂肪酸氧化。

Abstract:

To investigate the effect of cAMP on fatty acid oxidation in skeletal muscle cells and elucidate the mechanism of the effect of cyclic AMP(cAMP) on fatty acid oxidation in skeletal muscle. Methods The primary muscle cells(PMC) and C2C12 cells were treated with α-MSH,cAMP analogs Bt2cAMP,8-BrcAMP and cAMP antagonist respectively,and they were divided into α-MSH group,cAMP agonists group,cAMP antagonist plus α-MSH group,and control group was set up at the same time.The fatty acid oxidation was measured by quantitating the production of 3H2O from (9,10 -3H) palmitate. Melanocortin receptor(MCR) subtypes in the muscle and C2C12 cells were measured by RT-PCR. Results The fatty acid oxidation was significantly increased in cAMP analogs group and α-MSH group compared with control group(P<0.05),and the fatty acid oxidation in cAMP antagonist plus α-MSH group was significantly reduced compared with α-MSH group(P<0.05),and there was no significant difference compared with control group(P>0.05). Conclusion α-MSH and cAMP analogs significantly increase the fatty acid oxidation,α-MSH might be binding with MCR and they increase fatty acid oxidation in skeletal muscle through cAMP signal transduction pathway.

Key words: cAMP, α-melanocyte stimulating hormone, fatty acid oxidation

中图分类号:

R589.2

金勇君, 杨美子, 张凌云, 王秀云, 邢绍芝. 促黑素促进骨骼肌细胞脂肪酸氧化中cAMP的作用[J]. J4, 2010, 36(6): 1043-1046.

JIN Yong-Jun, YANG Mei-Zi, ZHANG Ling-Yun, WANG Xiu-Yun, XING Shao-Zhi. Effect of cAMP on fatty acid oxidation in skeletal muscle cells by α-MSH[J]. J4, 2010, 36(6): 1043-1046.