关键词: 角膜基质, 白细胞介素1α

Abstract: Objective To investigate the effects of hydrocortisone on degradation of keratocyte induced by interleukin-1α(IL-1α). Methods The keratocyte-collagen gels were incubated with plasminogen (0.06 μg•L^-1) and different concentrations of IL-1α（0，0.1,1.0, and 10.0 μg•L^-1） or interleukin-1α (10 μg•L^-1) and different concentrations of hydrocortisone(0,1,10, and 100 mmol•L^-1) or in MEM culture medium separately 24 h later, to evaluate the collagen degradation level, and the amount of hydroxyproline(HYP) were measured. Results In the presence of plasminogen, the amount of HYP was increased with the increasing of IL-1α concentration.There were significant differences（P<0.025 or P<0.05）between 0.1,1.0,and 10.0 μg•L^-1 IL-1α groups and control group（0 μg•L^-1）,between 1.0,10.0 μg•L^-1 groups and 0.1 μg•L^-1 group,between 10.0 μg•L^-1 group and 1.0 μg•L^-1 group. In the presence of plasminogen and IL-1α(10 μg•L^-1), the amount of HYP was increased with the increasing of hydrocortisone concentration. There were significant differences（P<0.025 or P<0.05）between 1,10,100 mmol•L^-1 hydroxyproline groups and control group (0 mmol•L^-1), between 10,100 mmol•L^-1 groups and 1 mmol•L^-1 group，between 100 mmol•L^-1 group and 10 mmol•L^-1 group. Conclusion Hydrocortisone can inhibit keratocyte-collagen degradation induced by IL-1α.

Key words: corneal stroma, interleukin-1α