关键词: 髓样, 慢性, 融合蛋白质类, bcr-abl, 脱氧腺苷类, 细胞凋亡

Abstract: Objective To study the effects of bcr/abl antisense oligodeoxynucleotide (ASON) on K562 cell growth. Methods Eighteen nucleotides were used as target genes in bcr/abl mRNA mixed area, by different bcr/abl cDNA sequence type analysis, 18 pdy ASON fragments and 18 poly non-sense ASON fragments for special sequence control were synthesized. Incubating K562 cells with ASON or non-sense ASON fragments for a mean time, while a blank control was set up. Cytomorphology, the ³H-TdR mixed rate and the expressions of bcr/abl mRNA and P210 protein were detected. Results ASON had different inhibitory rates on cell proliferation at 3.5-30.0 μmol•L^-1. The action was smaller when ASON concentration was less than 10.0 μmol•L^-1. While the action was more significant when the concentration was larger than 10.0 μmol•L^-1. This action depends on concentration and time. But non-sense ASON and control weren′t distinct different. At the meantime, ASON made the ³H-TdR mixed rate decrease and the expression levels of bcr/abl and P210 were down-regulated. Conclusion These results show that ASON hinders bcr/abl mRNA transcription and P210 protein translation, inhibits cell proliferation and promotes apoptosis on gene levels.

Key words: myeloid, chronic, fusion proteins, bcr-abl, deoxyadenosines, apoptosis