关键词: 毒性, 尿酸, 血液, 腺苷脱氨酶, 黄嘌呤氧化酶

Abstract: Objective To investigate the effects of heroin on plasma uric acid and purine nucleotides catabolism in rat spleen by testing the activities of adenosine deaminase (ADA) and xanthine oxidase (XOD). Methods Rats were administrated with heroin by intraperitoneal injection with increasing dose to develop addiction models. Fifty male Wistar rats were divided into five groups (n=10): control, 3 d adiminigstrationg group, 9 d adiminigstrationg group, 3 d withdrawal group and 8 d withdrawal group. The uric acid concentration in plasma and the activities of ADA and XOD in rat spleen were determined. Results The concentrations of uric acid in plasma in heroin administration groups were significantly increased compared with control group (P<0.01). Compared with 9 d administration groups, the concentrations of uric acid in withdrawal groups were not decreased apparently (P>0.05). The activities of ADA in spleen in heroin administration groups were significantly increased compared with control group （P<0.05）.It was decreased apparently in 8 d withdrawal group compared with 9 d administration group. The activity of XOD in spleen in 9 d administration group was increased apparently compared with control group（P<0.05）, there were no apparently differences between withdrawal groups and 9 d administration group （P＞0.05）, but in the withdrawal groups, the XOD in spleen had taken on a decreased tendence. Conclusion Administration of heroin can enhance the activities of ADA and XOD and promote the purine nucleotides catabolism in spleen.

Key words: toxicity, uric acid, blood, adenosine deaminase, xanthine oxidase