不同剂量低能量激光照射对体外人成骨样细胞增殖和分化的影响

J4 ›› 2009, Vol. 35 ›› Issue (6): 1123-1126.

不同剂量低能量激光照射对体外人成骨样细胞增殖和分化的影响

李秋实¹, 张天夫², 周延民¹

1.吉林大学口腔医院种植科,吉林长春 130041；2.吉林大学中日联谊医院口腔科|吉林长春 130033

收稿日期:2009-06-30 出版日期:2009-11-28 发布日期:2009-11-28
通讯作者: 周延民 E-mail:zhouym62@126.com
作者简介:李秋实（1981-）|女|黑龙江省鹤岗市人|医师|在读医学博士|主要从事口腔种植学研究。
基金资助:
吉林省计划委员会科研基金资助课题（2004科技字0727）

Effect of low level laser irradiation with different doses on proliferation and differentiation of human osteoblast-like cells in vitro

LI Qiu-Shi¹, ZHANG Tian-Fu², ZHOU Yan-Min¹

1.Department of Implantology,Stomatology Hospital,Jilin University,Changchun 130041,China;2. Department of Stomatology,China-Japan Union Hospital,Jilin University,Changchun 130033,China

Received:2009-06-30 Online:2009-11-28 Published:2009-11-28

摘要/Abstract

摘要：

目的:探讨不同剂量低能量激光(LLL)照射对人成骨样细胞增殖和分化的影响，为LLL的临床应用提供实验依据。方法：对数生长期人成骨细胞MG63随机分为3组，采用Ga-Al-As半导体激光器（808 nm,66 mW）进行照射，照射组1的剂量为1 J?cm-2，照射组2的剂量为 3 J?cm-2，对照组不进行激光照射。采用MTT法检测细胞增殖活力，免疫组化法检测细胞分化的标志骨桥蛋白（OPN）和Ⅰ型胶原蛋白（Coll Ⅰ）的表达情况。结果：与对照组比较，照射组1的细胞增殖活力和OPN、Coll Ⅰ表达量差异无显著性(P＞0.05)；24和48 h照射组2的细胞增殖活力明显高于对照组(P＜0.05)，且24 h时OPN、Coll Ⅰ表达量明显高于对照组(P＜ 0.05)。结论：3 J?cm-2剂量的低能量激光能促进人成骨样细胞的增殖和分化，可能促进骨修复。

关键词: 低能量激光；成骨样细胞；骨桥蛋白；胶原Ⅰ型

Abstract:

Objective
To investigate the effect of low level laser （LLL）irradiation with different doses on proliferation and differentiation of human osteoblast-like cells,and provide the experimental basis for clinical application of LLL.Methods Human osteoblast-like cells MG63 were irradiated by Ga-Al-As diode laser (808 nm,66 mW) with doses of 1 J?cm-2 and 3 J?cm-2 after being seeded into multi-well plates.Untreated cells were used as controls.The proliferation of MG63 was quantified by colorimetric MTT (dimethylthiazol tetrazolium bromide) assay.The expressions of the marker of differentiation osteopontin （OPN） and collagen Ⅰ （Coll Ⅰ） were observed by immunohistochemistry assay.Results There was no difference of proliferation and expressions of OPN and Coll Ⅰ 　between the laser group with dose of 1 J?cm-2 and control group(P>0.05). The laser group with the dose of 3 J?cm-2 showed more increase in cell survival at 24 and 48 h after being seeded as compared with control group(P<0.05).Moreover,the expressions of OPN and Coll Ⅰ in the laser group with dose of 3 J?cm-2 was also higher than those in control group (P<0.05).
Conclusion LLL irradiation with the dose of 3 J?cm-2 may promote the proliferation and differentiation of human osteoblast-like cells,which might improve bone healing.

Key words: low level laser;osteoblast-like cells;osteopontin;collagen , type Ⅰ

中图分类号:

R329.2

李秋实, 张天夫, 周延民. 不同剂量低能量激光照射对体外人成骨样细胞增殖和分化的影响[J]. J4, 2009, 35(6): 1123-1126.

LI Qiu-Shi, ZHANG Tian-Fu, ZHOU Yan-Min. Effect of low level laser irradiation with different doses on proliferation and differentiation of human osteoblast-like cells in vitro[J]. J4, 2009, 35(6): 1123-1126.

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