体外定向诱导人胎盘源间充质干细胞向内皮细胞分化

J4 ›› 2010, Vol. 36 ›› Issue (3): 500-504.

体外定向诱导人胎盘源间充质干细胞向内皮细胞分化

刘志辉¹|周延民²|王博蔚³|朱镇⁴|刘春丽⁵

1.吉林大学口腔医院修复科|吉林长春 130021；2. 吉林大学口腔医院种植科|吉林长春 130021；3.吉林大学第二医院妇产科| 吉林长春 130041；4.江苏省镇江市口腔医院修复科|江苏镇江 212002；5. 吉林大学口腔医院口腔颌面外科|吉林长春 130021

出版日期:2010-05-28 发布日期:2010-05-28
通讯作者: 周延民（Tel:0431-88796018，E-mail: starwbw@email.jlu.edu.cn）；刘春丽(Tel:0431-88796024,E-mail:wangbowei2009@hotmail.com） E-mail: starwbw@email.jlu.edu.cn wangbowei2009@hotmail.com
作者简介:刘志辉（1975-）|男|吉林省吉林市人|主治医师|医学博士|博士后在站|主要从事整形与外科重建研究。
基金资助:
吉林省科技厅青年科研基金资助课题（20080165）

Orientation differentiation of human placenat derived mesenchymal-like stem cells into endothelial cells in vitro

LIU Zhi-Hui¹, ZHOU Yan-Min², WANG Bo-Wei³, ZHU Tian⁴, LIU Chun-Li⁵

1.Department of Orthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;2.Department of Implant,Stomatology Hospital,Jilin University,Changchun 130021,China;3.Department of Obstetrics and Gynecology,Second Hospital,Jilin University,Changchun 130041,China;4. Department of Orthodontics,Stomatology Hospital of Zhenjiang,Zhenjiang 212002,China;5.Department of Oral and Maxillofacial Surgery,Stomatology Hospital,Jilin University,Changchun 130021,China

Online:2010-05-28 Published:2010-05-28

摘要/Abstract

摘要：

目的：探讨人胎盘源间充质干细胞（HPMSCS）在特定环境诱导下向内皮细胞分化的潜能，为其促进创伤愈合提供理论依据。方法：在患者知情同意的情况下，从足月分娩产妇娩出的胎盘组织中分离培养HPMSCS，通过倒置显微镜观察其生物学形态，利用流式细胞术检测其表面标志，并通过成骨诱导鉴定及成脂肪诱导鉴定确定其多向分化潜能。对鉴定后的HPMSCS在体外通过内皮细胞诱导液使之向内皮细胞分化，并对诱导后细胞进行细胞化学荧光染色，检测内皮细胞特异性Ⅷ因子（vWF）的表达；采用流式细胞术检测诱导后细胞表面标志，观察是否具有内皮细胞表型。结果：HPMSCS在培养基中持续培养后,形态由成纤维细胞样转变为类似内皮细胞的铺路石样结构。成熟内皮细胞特异的表面标志vWF为阳性表达。流式细胞术检测结果显示：CD31及CD34均有表达。结论：本诱导方案能够诱导HPMSCS出现内皮细胞表型,提示HPMSCS在体外特定条件下具有定向分化为内皮细胞的潜能。

关键词: 人胎盘源间充质干细胞；内皮细胞；诱导分化

Abstract:

Abstract:Objective To approach the potential of differentiation of human placenat derived mesenchymal-like stem cells(HPMSCS )into endothelial cells in vitro.Methods With the agreement of the patients,the HPMSCs were isolated and cultivated from the placenta of the term delivery parturients.The biomorphological changes were observed under inverted microscope.The surface marker was detected by flow cytometry,and through osteoblastic inducing identification and lypoblastic inducing identification, the multi-directional differentiation potential was defined.The HPMSCs were treated with endothelial cell inducing fluid in vitro and induced to differentiate into endothelial cells, and then fluorescent staining was used to dectect the expression of endothelial cell specific factor Ⅷ (vWF factor).The cell surface marker was detected by flow cytometry after induction.The endothelial cell phenotypes were observed.Results The shape of the cultured HPMSCs changed from fibroblast-like cells to endothelial-like cells.The expression of the surface marker-vWF was positive in induced HPMSCs,the expressions of CD31 and CD34 were also positive.Conclusion HPMSCS can be induced to appear the phenotypes of endothelial cells by this method,which indicates that HPMSCS have the ability to differentiate into endothelial cells in definite conditions in vitro.

Key words: human placenat derived mesenchymal-like stem cells;endothelial cells;induction and differentiation

中图分类号:

Q813

刘志辉, 周延民, 王博蔚, 朱镇, 刘春丽. 体外定向诱导人胎盘源间充质干细胞向内皮细胞分化[J]. J4, 2010, 36(3): 500-504.

LIU Zhi-Hui, ZHOU Yan-Min, WANG Bo-Wei, ZHU Tian, LIU Chun-Li. Orientation differentiation of human placenat derived mesenchymal-like stem cells into endothelial cells in vitro[J]. J4, 2010, 36(3): 500-504.