关键词:  肝纤维化；抗肝纤冲剂；Ⅰ型胶原蛋白

Abstract:

Objective  To explore the influence of Kangganxian Chongji on the expression of the hepatic collagenⅠ(Col Ⅰ) in the mice with hepatic fibrosis and clarify its theraputical function. Methods 75 female Balb/c mice  were randomly divided into 5 groups (n=15):normal control group,hepatic fibrosis model group,Colchicine group, low dosage therapeutic group and high dosage therapeutic group of Kangganxian Chongji. The hepatic fibrosis model mice were induced by intraperitoneal injection of pig serum respectively (0.5 mL/once,once a week,total 7 weeks) except for normal control group. At the same time the mice in therapeutic group were treated with Colchicine or Kangganxian Chongji once a day,lasted for 7 weeks.The mice were killed and the livers were took from the mice at the end of the 7 th week. The liver tissue slices were made and dyed with HE,and then the pathological changes of the mouse liver were observed  under microscope.The mRNA expression of Col Ⅰa2 and protein expression of  Col Ⅰa1 in the liver tissues were detected with real time PCR and Western blotting respectively.Results The pathological result indicated that the inflammation of liver tissue in Kangganxian Chongji group was alleviated  compared with model group.In low dosage Kangganxian Chongji group,the expression of Col Ⅰα2　 mRNA in liver tissue was markedly reduced compared with model group (P<0.01);at the same time,the expression of ColⅠα1 protein in liver tissue was markedly reduced according to Western blotting analysis compared with model group.Conclusion Kangganxian Chongji can obviously decrease the expression of ColⅠ protein of the liver tissue in the mice with hepatic fibrosis,and the results suggest that Kangganxian Chongji has obvious therapeutic function on hepatic fibrosis.

Key words: hepatc fibrosis;Kangganxian Chongji;collagenⅠ