关键词: 白细胞介素12, 基因疗法, 腹水

Abstract: Objective To evaluate the antitumor efficiency of IL-12 gene mediated by hydrodynamic injection in mouse models of malignant ascites.Methods To prepare the malignant ascites model,H₂₂ hepatoma cells were injected into peritoneal cavity of mice.The mice were divided into 3 groups.Plasmid pCMV-mIL-12,pCMVβ and saline were respectively administered into mice by hydrodynamic injection 3 d after H₂₂ cells injection.Thereafter,the blood was collected at indicated time points.The expressions of IL-12 and IFN-γ and NO in serum were determined.Three mice were sacrificed 14 d after treatment.The volume of ascites was measured.The cells in ascites were stained with hematoxylin and eosin or AO/EB.The other mice were kept to monitor the survival.Results Compared with mice treated with pCMVβ or saline,the survival of mice treated with pCMV-mIL-12 was obviously prolonged (P<0.01).The significant elevation of IL-12 and IFN-γ and NO levels in serum were detected(P<0.01,P<0.05) and less alive tumor cells(P<0.01),more apoptotic cells (P<0.01) were observed in mice treated with pCMV-mIL-12. Conclusion Hydrodynamic injection-mediated IL-12 gene transfer can prolong the survival of mice bearing malignant ascites by decreasing the production of hemorrhagic ascites and inducing apoptosis of tumor cells.

Key words: interleukin-12, gene therapy, ascites