IL-10和IL-12检测在结核性和恶性胸腔积液鉴别诊断中的应用

doi:10.13481/j.1671-587x.20170423

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 782-786.doi: 10.13481/j.1671-587x.20170423

IL-10和IL-12检测在结核性和恶性胸腔积液鉴别诊断中的应用

刘红梅¹, 初喆², 田锐³, 唐颖¹

1. 吉林大学第一医院呼吸内科, 吉林长春 130021;
2. 吉林大学第一医院急诊内科, 吉林长春 130021;
3. 空军航空大学门诊部, 吉林长春 130022

收稿日期:2016-08-17 出版日期:2017-07-28 发布日期:2017-08-01
通讯作者: 唐颖,主治医师(Tel:0431-88783442,E-mail:tang_ying@jlu.edu.cn) E-mail:tang_ying@jlu.edu.cn
作者简介:刘红梅(1969-),女,吉林省长春市人,主管护师,主要从事呼吸内镜在呼吸系统疾病诊断和治疗中应用的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20110489）；吉林省科技厅医药产业发展专项资金项目资助课题（20130727040YY）；吉林大学白求恩培育项目资助课题（2015328）

Application of IL-10 and IL-12 detection in differential diagnosis of tuberculous and malignant pleural effusion

LIU Hongmei¹, CHU Zhe², TIAN Rui³, TANG Ying¹

1. Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, China;
2. Department of Emergency, First Hospital, Jilin University, Changchun 130021, China;
3. Department of Out-patients, Aviation University of Air Force, Changchun 130022, China

Received:2016-08-17 Online:2017-07-28 Published:2017-08-01

摘要/Abstract

摘要： 目的：通过检测结核性和恶性胸腔积液患者血清和胸腔积液中白细胞介素10（IL-10）和白细胞介素12（IL-12）的水平，探讨二者对于鉴别结核性和恶性胸腔积液的价值。方法：选取住院未经治疗的渗出性胸腔积液患者48例，根据病因分为结核性胸腔积液组25例和恶性胸腔积液组23例。采用流式微球阵列法（CBA）检测2组患者血清和胸腔积液中IL-10和IL-12的水平，比较2组患者IL-10、IL-12水平和IL-12/IL-10比值的差异；通过受试者工作特征（ROC）曲线分析上述指标与胸水脱落细胞和腺苷脱氢酶（ADA）在鉴别结核性和恶性胸腔积液中的作用。结果：结核性胸腔积液组和恶性胸腔积液组患者血清中IL-10、IL-12水平和IL-12/IL-10比值比较差异无统计学意义（P> 0.05），恶性胸腔积液组患者胸腔积液中IL-12水平和IL-12/IL-10比值明显低于结核性胸腔积液组（P< 0.01）。利用胸腔积液IL-12水平鉴别结核性和恶性胸腔积液的ROC曲线下面积为0.984，明显高于IL-12/IL-10比值（0.744）、脱落细胞（0.804）和ADA（0.911）。结论：胸腔积液中IL-12水平检测有助于结核性和恶性胸腔积液的鉴别诊断，且标本容易获取，值得临床上推广应用。

关键词: 结核性胸腔积液, 白细胞介素10, 恶性胸腔积液, 白细胞介素12

Abstract: Objective: To investigate the role of interleukin-10 (IL-10) and IL-12 in the differential diagnosis of tuberculous and malignant pleural effusion through measuring the serum and pleural effusion IL-10 and IL-12 levels of tuberculous and malignant pleural effusion patients.Methods: Forty-eight inpatients with exudative pleural effusion who didn't receive any treatment before were selected. According to the pathogeny, the patients were divided into tuberculous pleural effusion group (n=25) and malignant pleural effusion group (n=23). The levels of IL-10 and IL-12 in peripheral blood and pleural effusion of the patients in two groups were detected by Cytometric Bead Array (CBA). The differences in IL-10, IL-12 levels and IL-12/IL-10 ratio were compared between two groups. The ROC curves of the above indexes, cast-off and adenosine deaminase (ADA) were used to compare the effects in differential diagnosis of tuberculous and malignant pleural effusion.Results: There were no statistical differences in the levels of serum IL-10, IL-12 and IL-12/IL-10 ratios of the patients between tuberculous and malignant pleural effusion groups (P>0.05),and the IL-12 level in pleural effusion and the IL-12/IL-10 ratio of the patients in malignant pleural effusion group were lower than those in tuberculous pleural effusion group (P<0.005).The area under the ROC curve for the levels of IL-12 in pleural effusion (0.984) was higher than those for the ratio of IL-12/IL-10, cast-off and ADA (0.744, 0.804 and 0.911, respectively) in the differential diagnosis of tuberculous and malignant pleural effusion.Conclusion: The detection of IL-12 levels in pleural effusion is helpful for the differential diagnosis of tuberculous and malignant pleural effusion, and the pleural effusion is easy to obtain, therefore the detection of IL-12 in pleural effusion is worth to be widely applied in clinic.

Key words: interleukin-10, interleukin-12, tuberculous pleural effusion, malignant pleural effusion

中图分类号:

R561.1

刘红梅, 初喆, 田锐, 唐颖. IL-10和IL-12检测在结核性和恶性胸腔积液鉴别诊断中的应用[J]. 吉林大学学报(医学版), 2017, 43(04): 782-786.

LIU Hongmei, CHU Zhe, TIAN Rui, TANG Ying. Application of IL-10 and IL-12 detection in differential diagnosis of tuberculous and malignant pleural effusion[J]. Journal of Jilin University Medicine Edition, 2017, 43(04): 782-786.

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IL-10和IL-12检测在结核性和恶性胸腔积液鉴别诊断中的应用

Application of IL-10 and IL-12 detection in differential diagnosis of tuberculous and malignant pleural effusion

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