恶性胶质瘤细胞死亡诱导信号复合体表达与TRAIL诱导凋亡的关系

J4 ›› 2012, Vol. 38 ›› Issue (5): 904-906.

恶性胶质瘤细胞死亡诱导信号复合体表达与TRAIL诱导凋亡的关系

于洪泉¹, 张宇¹, |金宏², 赵东海²,杨淑艳², 齐玲²

1.吉林大学第一医院神经外科,吉林长春130021|2.吉林医药学院病理学教研室,吉林吉林132013

收稿日期:2012-06-18 出版日期:2012-09-28 发布日期:2012-09-28
通讯作者: 齐玲（Tel：0431-88782331，E-mail：qiling1718@163.com） E-mail:qiling1718@163.com
作者简介:于洪泉（1974-）|男|吉林省长春市人|主治医师|医学博士|主要从事脑肿瘤研究。
基金资助:
吉林省科技厅自然科学基金资助课题（202015242）；吉林省教育厅科研基金资助课题（2012330）；吉林医药学院科研基金资助课题（201101）；吉林省吉林市科技发展计划项目资助课题（201233128）

Relationship between |expression of death-inducing signalingcomplex and apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand |in malignant glioma cells

YU Hong-quan¹,ZHANG Yu¹,JIN Hong²,ZHAO Dong-hai²,YANG Shu-yan²,QI Ling²

1.Department of Neurosurgery,First Hospital,Jilin University,Changchun 130021,China|2.Department of Pathology,Jilin Medical College,Jilin 132013,China

Received:2012-06-18 Online:2012-09-28 Published:2012-09-28

摘要/Abstract

摘要：

目的：通过研究恶性胶质瘤细胞中死亡诱导信号复合体(DISC)与肿瘤坏死因子相关凋亡诱导配体（TRAIL）诱导凋亡的关系，初步探讨TRAIL诱导凋亡抵抗的机制。方法：分离恶性胶质瘤组织，获得和培养原代胶质瘤细胞，用100 μg/L TRAIL作用后采用酸性磷酸酶法检测细胞凋亡水平；Western blotting 法检测细胞表达死亡诱导信号复合体的水平。结果：获得3株（GC417、GC321、GC125）原代恶性胶质瘤细胞；3株细胞对TRAIL诱导凋亡的敏感程度不同［GC321（0.12±0.01 vs 0.51±0.02）和GC125（0.22±0.01 vs 0.36±0.01）］，对TRAIL诱导凋亡作用敏感，与对照组比较差异有统计学意义（P<0.01）；而GC417（0.24±0.01 vs 0.23±0.02）对TRAIL诱导凋亡不敏感。Western blotting法检测结果显示，死亡诱导信号复合体表达不同，GC321和GC125表达增高，GC417表达减少。结论：不同来源的原代培养恶性胶质瘤细胞对TRAIL诱导凋亡的反应不同，死亡诱导信号复合体表达也不同，死亡诱导信号复合体表达的减少可能与凋亡抵抗的发生密切相关。

关键词: 胶质瘤；死亡诱导信号复合体；肿瘤坏死因子相关凋亡诱导配体；细胞凋亡

Abstract:

Abstract:Objective To discuss the relationship between the expression of death-inducing signaling complex (DISC) and apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) in malignant glioma cells,and to explore the mechanism of resistance of TRAIL initiated apoptosis. Methods The primary cultured cells were isolated from human malignant glioma tissues,and the level of apoptotsis was detected by acid phosphatase assay after the cells treated with different doses of TRAIL; and the level of DISC protein was determined by Western blotting.Results The primary malignant glioma cells GC417,GC321 and GC125 were isolated and cultivated,and the sensitivities of the cells to TRAIL were different,GC321 (0.12±0.01 vs 0.51±0.02) and GC125 (0.22±0.01 vs 0.36±0.01) were sensitive to TRAIL,there were significant differences compared with control group(P<0.01);but GC417 (0.24±0.01 vs 0.23±0.02 ) was resistant to TRAIL.The results of Western blotting showed that the expressions of DISC proteins were various,and the expressions of GC321 and GC125 were increased,and the expression of GC417 was decreased.Conclusion The responses are different according to the different primary malignant glioma cells to TRAIL-induced apoptosis,and the expressions of DISC proteins are either different; there is relationship between the decreasing of the expressions of DISC proteins and the resistance of TRAIL initiated apoptosis.

Key words: glioma, death-inducing signaling complex, tumor necrosis factor related apoptosis-inducing ligand, apoptosis

中图分类号:

R739.4

于洪泉, 张宇, 金宏, 赵东海,杨淑艳, 齐玲. 恶性胶质瘤细胞死亡诱导信号复合体表达与TRAIL诱导凋亡的关系[J]. J4, 2012, 38(5): 904-906.

YU Hong-quan,ZHANG Yu,JIN Hong,ZHAO Dong-hai,YANG Shu-yan,QI Ling. Relationship between |expression of death-inducing signalingcomplex and apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand |in malignant glioma cells[J]. J4, 2012, 38(5): 904-906.