化学去细胞肌肉组织工程支架与大鼠脊髓的生物相容性

J4 ›› 2009, Vol. 35 ›› Issue (5): 801-804.

化学去细胞肌肉组织工程支架与大鼠脊髓的生物相容性

薛辉¹, 陈东^1,2, 张秀英^1,3, 刘颖¹

1.吉林大学基础医学院组织学与胚胎学教研室,吉林长春130021;2.广东医学院组织学与胚胎学教研室,广东湛江524023;3.吉林大学护理学院基础护理教研室,吉林长春 130021

收稿日期:2009-03-24 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 陈东 E-mail:nbumschendong@yahoo.com.cn
作者简介:薛辉(1978-)|男|吉林省长春市人|讲师,医学博士,主要从事干细胞增殖和分化研究。
基金资助:
国家自然科学基金资助课题(30970739);教育部高等学校博士点专项科研基金资助课题(20030183048)

Biocompatibility of chemically extracted acellular muscle grafts as biomatrices in experimental spinal cord injury in rats

XUE Hui¹, CHEN Dong^1,2, ZHANG Xiu-Ying^1,3, LIU Ying¹

1. Department of Histology and Embryology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2 .Department of Histology and Embryology,Guangdong Medical College,Zhanjiang 524023,China;3. Department of Fundamental Nursing,School of Nursing,Jilin University,Changchun 130021,China

Received:2009-03-24 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：探讨化学去细胞肌肉支架与大鼠脊髓的生物相容性，阐明化学去细胞肌肉作为神经组织工程支架治疗脊髓损伤的优势。方法：将36只成年雄性大鼠随机分成2组：植入组和空白对照组。制备脊髓半横断损伤模型后，造模成功大鼠分别于脊髓缺损处植入化学去细胞肌肉或不做处理。每组6只分别于术后1、2和4周处死取材。免疫组织化学染色观察不同时间点损伤脊髓处的ED-1阳性巨噬细胞。取术后4周切片，Holmes镀银染色观察损伤脊髓的再生轴突，免疫组织化学染色观察损伤脊髓的GFAP阳性星形胶质细胞，碱性磷酸酶组织化学染色观察支架中的再生血管。计数ED-1阳性细胞和再生轴突。结果：空白对照组损伤处无轴突再生；其炎症反应在术后1周时最强，之后逐渐减弱；星形胶质细胞在损伤处或空洞周围呈密集多层排列。而植入组化学去细胞肌肉中再生轴突数为613.17±154.96，再生轴突排列方式规则；其炎症反应趋势与空白组相同，未见异物排斥反应；星形胶质细胞成排平行长入支架；化学去细胞肌肉中血管再生良好。结论：化学去细胞肌肉支架与脊髓具有良好的生物相容性，提示化学去细胞肌肉可作为治疗脊髓损伤的组织工程支架。
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关键词: 脊髓损伤;去细胞肌肉;移植;组织工程;大鼠,Wistar

Abstract:

Abstract:Objective To evaluate the biocompatibility of chemically extracted acellular muscle grafts (CEAM) and to demonstrate its advantages as tissue engineering scaffold after transplantation into the spinal cords of adult rats.Methods Thirty-six male rats were randomly assigned to control group and CEAM group, and underwent spinal cord lateral hemisection.After preparation of successful models,treatment consisted of application of CEAM into the lesion gap,which was left empty in control group.Six rats in each group were killed 1,2 and 4 weeks after induction of the injury.Sections were stained for quantification of microglia/macrophages using ED-1 on week 1,week 2 and week 4.Additionally,Holmes’ silver staining method was chosen to detect axonal regeneration,glial fibrillary acidic protein (GFAP) staining for detection of astrocytes in glial scars,and alkaline phosphatase staining for vascularisation on day 28. Results In control group,no definite axonal outgrowth into the lesion was found.The inflammatory response was most pronounced on day 7 and a dense non-oriented accumulation of astrocytes could be found at the edge of the lesion cavity in control group.In CEAM group,the number of regenerating axons in the scaffolds was 613.17±154.96,and they grew into the grafts in a strikingly organized fashion. The change of inflammatory response in CEAM group was the same as that in control group and there was no sign of the foreign body reaction induced by CEAM.In rats of CEAM group the astrocytes grew into the graft in a diffuse linear array.Vascularisation of the grafts was also confirmed.Conclusion CEAM is biocompatible with spinal cord,and it indicates that CEAM is a kind of good tissue engineering scaffold for the repair of spinal cord injury.
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Key words: spinal cord injury；acellular muscle；transplantation；tissue engineering;rats,Wistar

中图分类号:

R318.19

薛辉, 陈东, 张秀英, 刘颖. 化学去细胞肌肉组织工程支架与大鼠脊髓的生物相容性[J]. J4, 2009, 35(5): 801-804.

XUE Hui, CHEN Dong, ZHANG Xiu-Ying, LIU Ying. Biocompatibility of chemically extracted acellular muscle grafts as biomatrices in experimental spinal cord injury in rats[J]. J4, 2009, 35(5): 801-804.