HSP65-MUC1／HSP65抗体免疫复合物对人树突状细胞的活化作用

doi:国家自然科学基金资助课题

HSP65-MUC1／HSP65抗体免疫复合物对人树突状细胞的活化作用

任淑萍^1#, 包木胜¹,高新¹,于永利², 王丽颖^1*

1. 吉林大学基础医学院分子生物学教研室，吉林长春 130021；2. 吉林大学基础医学院免疫学教研室，吉林长春 130021

收稿日期:2005-01-15 修回日期:1900-01-01 出版日期:2005-03-28 发布日期:2005-03-28
通讯作者: 王丽颖

Activation and maturation of dendritic cells induced by HSP65-MUC1/HSP65 immune complex

REN Shu-ping^1#，BAO Mu-sheng¹，GAO Xin¹， YU Yong-li²，WANG Li-ying^1*

1. Department of Molecular Biology, School of Basic Medical Sciences, Jilin University,Changchun 130021, China；2. Department of Immunology, School of Basic Medical Sciences,Jilin University, Changchun 130021, China

Received:2005-01-15 Revised:1900-01-01 Online:2005-03-28 Published:2005-03-28
Contact: WANG Li-ying

摘要/Abstract

摘要： 目的：探讨免疫复合物能否诱导树突状细胞的活化与成熟。方法：用免疫复合物刺激来自人外周血经细胞因子IL-4和GM-CSF诱导的未成熟的树突状细胞, 观察免疫复合物对未成熟树突状细胞的活化与成熟的影响。结果：HSP65-MUC1/HSP65免疫复合物与HSP65-MUC1抗原或HSP65抗体相比较，能够显著地活化树突状细胞，使其表面的协同刺激分子CD86的表达显著上调，为激活细胞毒性T淋巴细胞提供第二信号，这一结果说明免疫复合物具有交叉递呈的作用；同时，加入免疫复合物的树突状细胞培养上清中IL-6和TNFα分泌量明显增加。结论：免疫复合物能够诱导树突状细胞的活化与成熟，免疫复合物具有交叉递呈的作用。

关键词: 树突细胞, 抗原抗体复合物, 交叉递呈, 免疫活化

Abstract: Objective To explore the activation and maturation of dendritic cells (DC) stimulated by immune complex. Methods Immune complex was added to the immature DC induced by IL-4 and GM-CSF from PBMC and the expressions of costimulatory molecules CD86 on the surface of DC and cytokines in the supernatant were detected. Results Compared with antigen or antibody, immune complex could significantly activate the DC by upregulating the expression of CD86 on the surface of DC which was the second signal for the activation of cytotoxic T lymphocytes and the secretions of IL-6 and TNFα. Conclusion Immune complex can induce the activation and maturation of DC.

Key words: dendritic cells, antigen-antibody complex, cross-presentation, immunocompetence

中图分类号:

任淑萍, 包木胜,高新,于永利, 王丽颖. HSP65-MUC1／HSP65抗体免疫复合物对人树突状细胞的活化作用[J]. J4, 2005, 31(2): 182-185.

REN Shu-ping，BAO Mu-sheng，GAO Xin， YU Yong-li，WANG Li-ying. Activation and maturation of dendritic cells induced by HSP65-MUC1/HSP65 immune complex[J]. J4, 2005, 31(2): 182-185.

[1]	林笑颖, 周明倩, 李海昌. 中性粒细胞胞外诱捕网在系统性红斑狼疮发病机制中作用的研究进展[J]. 吉林大学学报(医学版), 2018, 44(04): 886-890.
[2]	劳凤学，柳忠辉，刘端祺. Flt3配体在肿瘤免疫治疗中的应用[J]. J4, 2009, 35(2): 393-396.
[3]	杨蕾，霍锐，卜丽梅，杜珍武，邸军，张桂珍. 结肠癌细胞总RNA电穿孔法体外转染成熟树突状细胞[J]. J4, 2009, 35(2): 271-275.
[4]	翟妍，张震宇，乔宝丽，刘晋伟. 大鼠骨髓来源树突状细胞的分离与免疫学特征[J]. J4, 2007, 33(6): 952-955.
[5]	李杰，丁艳华，邵明柏，王冠军，金宁一. 转染IL-18基因慢性粒细胞白血病树突状细胞的抗白血病作用[J]. J4, 2007, 33(6): 1061-1065.
[6]	侯治富，郭楠，高申，郑德明，卜丽莎，高嵩，才华. 人外周血树突状细胞的诱导与鉴定[J]. J4, 2005, 31(5): 657-660.
[7]	万敏, 张培因, 王宣军, 吴秀丽, 卫红飞, 王燕媚, 于永利, 王丽颖，邓平，张慧杰. 热休克蛋白融合蛋白对人树突状细胞分化成熟的刺激作用[J]. J4, 2004, 30(6): 835-838.
[8]	邵国光，郭喜平，张勇，徐沛然，杨志广. 免疫抑制剂预处理对同种异体气管移植犬免疫耐受的影响[J]. J4, 2004, 30(6): 912-914.
[9]	陈玉丙，张红梅，刘林林，范洪学. 小鼠骨髓干细胞体外诱导成熟的树突状细胞对T细胞增殖的影响[J]. J4, 2004, 30(3): 350-353.