RNA干扰沉默STAT3基因表达对人胰腺癌细胞SW1990侵袭能力的影响

J4 ›› 2011, Vol. 37 ›› Issue (4): 636-640.

RNA干扰沉默STAT3基因表达对人胰腺癌细胞SW1990侵袭能力的影响

王葆春¹|孙杨柳²|宫晓光¹|费瑞³

1.海南省人民医院普外科|海南海口 570311；2.吉林省长春市中心医院药剂科|吉林
长春130051；3. 吉林大学白求恩医学院细胞生物学系|吉林长春 130021

收稿日期:2011-03-22 出版日期:2011-07-28 发布日期:2011-07-28
通讯作者: 费瑞（Tel:0431-85619473,E-mail: fayrui64@yahoo.com.cn） E-mail: fayrui64@yahoo.com.cn
作者简介:王葆春（1969-）|男|吉林省延吉市人|副主任医师|医学硕士|主要从事肝胆外科治疗的研究。
基金资助:
吉林省科技厅科技发展计划重点项目资助课题（20100920）；海南省卫生厅科研项目资助课题（琼卫2010-7）

Effects of STAT3 silenced with RNAi on invasiveness of
human pancreatic cancer cell line SW1990

WANG Bao-chun¹,SUN Yang-liu²,GONG Xiao-guang¹|FEI Rui³
Rui3

1.Department of General Surgery,People’s Hospital of Hainan,Haikou 570311,
China;2. Department of Pharmacy,Central Hospital of Changchun,Changchun 13005
1,China;3. Department of Cell Biology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China

Received:2011-03-22 Online:2011-07-28 Published:2011-07-28

摘要/Abstract

摘要：

目的：观察信号转导与转录因子3（STAT3）siRNA表达载体对胰腺癌细胞侵袭能
力的影响，并探讨其机制。方法：实验分为空白对照组、阴性对照组及STAT3 siRNA组。以人
胰腺癌细胞株SW1990作为研究对象，将合成的STAT3 siRNA转染SW1990细胞后，采用RT-PCR及
Western blotting检测转染前后STAT3 mRNA及蛋白的表达变化，Transwell法检测其对胰腺癌
细胞侵袭能力的影响，RT-PCR及Western blotting检测其对胰腺癌细胞MMP-2和MMP-9表达
的影响。结果：STAT3 siRNA转染胰腺癌细胞72 h后，STAT3 mRNA和蛋白表达水平均明显降低，
与空白对照组及阴性对照组比较，差异有统计学意义（P<0.05）；转染后胰腺癌细
胞的侵袭细胞数及MMP-2和MMP-9的表达显著减小，与阴性对照组及空白对照组比较，差异有统
计学意义（P<0.05）。结论：STAT3 siRNA能特异地阻断胰腺癌细胞中STAT3信号的活
化，并进一步通过下调MMP-2和MMP-9的表达从而抑制胰腺癌细胞的侵袭能力。

关键词: 胰腺肿瘤；信号转导及转录活化子3；RNA干扰；侵袭能力

Abstract:

Abstract:Objective To investigate the effects of signal transducer and activator of transcription 3 (STAT3) on the invasiveness of pancreatic cancer cell line SW1990 and its mechanism.Methods The SW1990 cells were divided into control group,negative group and siRNA group.The cells in siRNA group were transfected with STAT3 siRNA,the expressions of STAT3 mRNA and protein were assayed by RT-PCR and Western blotting;the invasive ability of cancer cells was detected by Transwell assay.The expressions of MMP-2 and MMP-9 of pancreatic cancer cells were detected by RT-PCR and Western blotting.Results After transfected with STAT3 siRNA for 72h,the expressions of STAT3 mRNA and protein were significantly decreased,and there were significant differences compared with control group and negative group (P<0.05).The number of invaded cells in siRNA group was decreased significantly compared with control group and negative group (P<0.05).At mRNA and protein levels,the expressions of MMP-2 and MMP-9 were significantly decreased in siRNA group,and there were significant differences compared with control group and negative group (P<0.05).Conclusion STAT3 siRNA could specifically block the activaty of STAT3 signal on SW1990 and also inhibit the invasiveness of pancreatic cancer cells by down-regulating the MMP-2 and MMP-9 expressions.

Key words: pancreatic neoplasms;signal trarsducer and activator of transcription 3;RNA interference;invasiveness

中图分类号:

R349.64

王葆春|孙杨柳|宫晓光|费瑞 . RNA干扰沉默STAT3基因表达对人胰腺癌细胞SW1990侵袭能力的影响[J]. J4, 2011, 37(4): 636-640.

WANG Bao-chun,SUN Yang-liu,GONG Xiao-guang,FEI Rui. Effects of STAT3 silenced with RNAi on invasiveness of
human pancreatic cancer cell line SW1990[J]. J4, 2011, 37(4): 636-640.