硫酸锌对UVB致HaCaT细胞损伤的拮抗作用

J4 ›› 2011, Vol. 37 ›› Issue (4): 646-650.

硫酸锌对UVB致HaCaT细胞损伤的拮抗作用

邢雷¹|潘奇正¹|李云²|时佳宏¹|李建会¹|任治兴¹|任淑萍¹

1. 吉林大学公共卫生学院卫生毒理学教研室|吉林长春 130021；2. 吉林大学中日联谊医院眼科|吉林长春 130033

收稿日期:2011-04-15 出版日期:2011-07-28 发布日期:2011-07-28
通讯作者: 任淑萍（Tel: 0431-85619133,E-mail: rensp@jlu.edu.cn） E-mail:rensp@jlu.edu.cn
作者简介:邢雷（1986-）|男|黑龙江省宁安市人|在读医学硕士|主要从事环境有害因子损害作用及其机制研究。
基金资助:
中华医学会欧莱雅基金资助课题（S-2007040803）；吉林大学大学生创新基金资助课题（2010A72111）

Effects of zinc sulfate against UVB damage on HaCaT cells

XING Lei¹,PAN Qi-zheng¹,LI Yun^2,SHI Jia-hong¹,LI Jian-hui¹,REN Zhi-xing¹,REN Shu-ping¹

1. Department of Hygiene Toxicology,School of Public Health,Jilin University,
Changchun 130021,China;2. Department of Ophthamology,China-Japan Union Hosptial,Jilin University,Changchun 130033,China

Received:2011-04-15 Online:2011-07-28 Published:2011-07-28

摘要/Abstract

摘要：

目的：探讨中波紫外线（UVB）造成HaCaT细胞损伤的机制及硫酸锌能否拮抗UVB对细胞的损害作用，为开发预防UVB损伤的药物提供理论依据。方法：将HaCaT细胞随机分为对照组、UVB组、加锌（Zn）组和Zn+UVB组。对照组不施加任何处理因素；UVB组细胞进行UVB照射；Zn组只加入终浓度为50 μmol·L^-1的硫酸锌；Zn+UVB组，加入硫酸锌24　h后，进行UVB照射。UVB照射时间为6 min，照射后24　h，收集各组细胞，用台盼蓝拒染法检测细胞存活率，TBA显色法检测细胞内丙二醛（MDA）水平，单细胞凝胶电泳技术检测各组细胞DNA的损伤情况。结果：与对照组比较，UVB组和Zn+UVB组HaCaT细胞存活率显著降低（P<0.05），MDA水平显著升高（P<0.05）；与UVB组比较，Zn+UVB组细胞存活率显著升高（P<0.05），MDA水平显著降低（P<0.05）。单细胞凝胶电泳检测结果显示，UVB组和Zn+UVB组HaCaT细胞均出现彗星样拖尾现象。与对照组比较，UVB组和Zn+UVB组细胞尾部DNA尾距、Olive尾距显著增大（P<0.05）；与UVB组比较，Zn+UVB组尾距、Olive尾
距显著减小，差异有统计学意义（P<0.05）。结论：UVB　能够导致HaCaT细胞存活率下降和MDA水平提高以及DNA损伤，而硫酸锌能够拮抗UVB对HaCaT细胞的损伤作用。

关键词: 中波紫外线；HaCaT细胞；DNA损伤；单细胞凝胶电泳；丙二醛

Abstract:

Abstract:Objective To study the antagonism of zinc sulfate on damage effect of ultraviolet B (UVB) on HaCaT cells,and to provide theoretical basis for development of drug to prevent UVB damage. Methods The HaCaT cells were cultivated in RPMI 1640 and randomly divided into control group,Zn group,UVB group and Zn+UVB group.Nothing was administrated to the cells in control group,the cells in UVB group were radiated by UVB,the cells in Zn group were administrated with 50 μmol·L^-1 ZnSO4 and the cells in Zn+UVB group were administrated with 50 μmol·L^-1 ZnSO4 24 h before UVB radiation.The UVB radiation time was 6 min,the cells in every group were collected 24 h after UVB radiation.The cell viability was measured by trypan blue exclusion assay,the MDA level was measured by TBA method,the DNA damage in HaCaT cells was me
asured by single cell gel electrophoresis(SCGE).Results Compared with control group,24 h after radiation,the cell viabilities in UVB group and Zn+UVB group were significantly decreased (P<0.05),the MDA levels were increased (P<0.05).Compared with UVB group,the cell viability in Zn+UVB group was significantly increased (P<0.05),the MDA level was decreased (P<0.05).The SCGE results revealed that the DNA fragmentation(comet) of the cells in UVB group and Zn+UVB group were induced by UVB. The tail extent moment and Olive tail moment of the cells in UVB group were significantly higher than those in Zn+UVB group (P<0.05).
Conclusion UVB can lead the decreasing of the viability of HaCaT cells,the increasing of MDA level and DNA damage,while zinc sulfuric can antagonize the damage effect of UVB on HaCaT cells.

Key words: ultraviolet , B;HaCaT cells;DNA damage;single cell gel electrophoresis;malonaldehyde

中图分类号:

R811.5

邢雷|潘奇正|李云|时佳宏|李建会|任治兴|任淑萍. 硫酸锌对UVB致HaCaT细胞损伤的拮抗作用[J]. J4, 2011, 37(4): 646-650.

XING Lei,PAN Qi-zheng,LI Yun,SHI Jia-hong,LI Jian-hui,REN Zhi-xing,REN Shu-ping. Effects of zinc sulfate against UVB damage on HaCaT cells[J]. J4, 2011, 37(4): 646-650.