山葡萄多酚对酒精慢性中毒大鼠氧化损伤的保护作用

J4 ›› 2009, Vol. 35 ›› Issue (4): 639-641.

山葡萄多酚对酒精慢性中毒大鼠氧化损伤的保护作用

戴维群¹|倪海镜¹|焦淑^萍2

1.吉林大学中日联谊医院药剂科|吉林长春 130033；2.北华大学医学院生物化学教研室|吉林吉林 132013

收稿日期:2009-03-03 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 倪海镜 E-mail:nihaij9966@126.com
作者简介:戴维群（1961-）|女|吉林省长春市人|主管药师|主要从事临床药学研究。
基金资助:
吉林省科技厅科研基金资助课题（200302-05）

Protective effects of |polyphenols of vitis amurensis Rupr on rat oxidative damage caused by chronic alcohol intakes

DAI Wei-Qun¹, NI Hai-Jing¹, JIAO Shu-Ping²

1. Department of Pharmacy Service|China-Japan Union Hospital| Jilin University,Changchun 130033|China;2.Department of Biochemistry,Medical College,Beihua University,Jilin 132013,China

Received:2009-03-03 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的:研究山葡萄多酚(PVAR)对酒精慢性中毒大鼠肝脏氧化损伤的保护作用,阐明PVAR防治酒精性肝病(ALD)的理论基础。方法:Wistar雄性大鼠40只，按体重区间随机分4组，每组10只：对照组，酒精组（33%酒精10 mL·kg^-1 灌胃），低、高剂量PVAR组（33%酒精10 mL·kg^-1 灌胃前，灌胃200和400 mg·kg^-1  PVAR）。连续实验8周后，检测大鼠肝脏组织丙二醛(MDA)、活性氧（ROS）、谷胱甘肽（GSH）含量、超氧化物歧化酶(SOD)活性、微粒体血红素氧化酶-1（HO-1）的活性以及血清谷丙转氨酶（GPT）、谷草转氨酶（GOT）含量。结果:与酒精组比较，低、高剂量PVAR组大鼠肝脏组织ROS、MDA明显降低(P<0.05)而GSH含量显著升高 (P<0.05,P<0.01)；血清GPT、GOT含量明显下降（P<0.05)，高剂量PVAR组SOD、HO-1活性明显升高（P<0.05）。结论:PVAR对酒精慢性中毒大鼠肝脏氧化损伤具有保护作用，其机制可能与诱导HO-1活性，清除自由基，抑制脂质过氧化反应有关。

关键词: 山葡萄多酚；酒精慢性摄入；氧化损伤

Abstract:

Objective
To study the protective effects of the polyphenols of vitis amurensis Rupr(PVAR) on oxidative damage in rats caused by chronic alcohol intakes.Methods Forty male Wistar rats were divided into 4 groups according rats’ weights: normal control group,alcohol injury group(the rats were treated with 10mL·kg^-1  33% alcohol via i.g);PVAR group(after ingesting 200 and 400 mg·kg^-1 PVAR,the male rats were treated with 10 mL·kg^-1 33% alcohol via i.g).After 8 weeks,the concentrations of malondialdehyde(MDA),reactive oxygen species(ROS),glutathione(GSH) and the activities of SOD and heme oxygenase-1(HO-1) in rat liver tissues were measured.The activities of serum glutamic-pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) in rats were measured.Results Compared with alcohol injury group,the concentrations of MDA,ROS,GPT and GOT were decreased significantly (P<0.05),and the GSH level was increased significantly(P<0.01) in PVAR groups.The activities of SOD,HO-1 in PVAR groups were increased significantly(P<0.05).Conclusion PVAR has protective effects on oxidative damage induced by chronic alcohol intakes and the effects are related to increasing HO-1 activity,scavenging oxygen free radicals and reducing lipid peroxidation.

Key words: polyphenols of vitis amurensis Rupr;chronic alcohol intakes;oxidative damage

中图分类号:

R965.1

戴维群, 倪海镜, 焦淑萍. 山葡萄多酚对酒精慢性中毒大鼠氧化损伤的保护作用[J]. J4, 2009, 35(4): 639-641.

DAI Wei-Qun, NI Hai-Jing, JIAO Shu-Ping. Protective effects of |polyphenols of vitis amurensis Rupr on rat oxidative damage caused by chronic alcohol intakes[J]. J4, 2009, 35(4): 639-641.