关键词: 细胞凋亡, 磷脂酰丝氨酸类, 流式细胞术

Abstract: To analyze the change of phosphotidyl serine(PS) in apheresis platelets(plts) during storage process,and to evaluate the quality of the plts during the storage period. MethodsThe apoptosis ligand Fas(CD95) was used to induce plts as apoptosis model,and by which the method to detect plts PS by flow cytometry(FCM) was established.Subsequently the resting PS expressive rate and the induced PS expressive rate of 30 apheresis plts,which induced by CD95,were determined using the FCM method established in storage process.ResultsAt the 1st,5th,7th,8th day of storage process,the PS expression of plts kept on increasing.The PS expressive rate of plts were 2.03%±0.91%,3.26%±1.86%,8.98%±4.60%,26.68%±21.28%,and 38.85%±26.43%,respectively,and there were significant differences among groups respectively(P<0.05).Under induced-operation of CD95,the PS induced-expressive rate of every storage group was obviously higher than that of 0 day group (P<0.01).The PS expression rate of plts had no difference between 7-day storage group and 0-day induced group by CD95 (P>0.05).ConclusionThe PS expression of apheresis plts increase significantly with the prolongation of storage time;Fresh plts has the ability of anti-apoptosis;Apheresis plts could keep normal function until storage 7 d.

Key words: apoptosis, phosphotidyl serine, flow cytometry